Rapid sequencing of the mycobacterium tuberculosis pncA gene for detection of pyrazinamide susceptibility
Date
2014
Journal Title
Journal ISSN
Volume Title
Publisher
American Society for Microbiology
Abstract
We developed a pyrazinamidase gene DNA-sequencing method to rapidly identify pyrazinamide resistance-causing mutations in GenoLyse-treated, smear-positive sputum specimens. The sensitivity and specificity were 90.9 and 100%, respectively, compared to those of MGIT drug susceptibility testing, after the exclusion of synonymous mutations and nonsynonymous mutations previously associated with susceptibility to pyrazinamide.
Description
CITATION: Streicher, E. M., et al. 2014. Rapid sequencing of the mycobacterium tuberculosis pncA gene for detection of pyrazinamide susceptibility. Journal of Clinical Microbiology, 52(11): 4056-4057. doi: 10.1128/JCM.02438-14.
The original publication is available at http://jcm.asm.org
The original publication is available at http://jcm.asm.org
Keywords
Mycobacterium tuberculosis -- Immunological aspects, Pyrazinamide susceptibility, Drug resistance, Sequencing
Citation
Streicher, E. M., et al. 2014. Rapid sequencing of the mycobacterium tuberculosis pncA gene for detection of pyrazinamide susceptibility. Journal of Clinical Microbiology, 52(11): 4056-4057. doi: 10.1128/JCM.02438-14.