Rapid sequencing of the mycobacterium tuberculosis pncA gene for detection of pyrazinamide susceptibility

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dc.contributor.authorStreicher, Elizabeth M.en_ZA
dc.contributor.authorMaharaj, Kashmeelen_ZA
dc.contributor.authorYork, Talitaen_ZA
dc.contributor.authorVan Heerden, Carelen_ZA
dc.contributor.authorBarnard, Marinusen_ZA
dc.contributor.authorDiacon, Andreasen_ZA
dc.contributor.authorMendel, Carl M.en_ZA
dc.contributor.authorBosman, Marlein E.en_ZA
dc.contributor.authorHepple, Juli A.en_ZA
dc.contributor.authorPym, Alexander S.en_ZA
dc.contributor.authorWarren, Robin M.en_ZA
dc.contributor.authorVan Helden, Paul D.en_ZA
dc.date.accessioned2016-06-20T07:50:53Z
dc.date.available2016-06-20T07:50:53Z
dc.date.issued2014
dc.descriptionCITATION: Streicher, E. M., et al. 2014. Rapid sequencing of the mycobacterium tuberculosis pncA gene for detection of pyrazinamide susceptibility. Journal of Clinical Microbiology, 52(11): 4056-4057. doi: 10.1128/JCM.02438-14.
dc.descriptionThe original publication is available at http://jcm.asm.org
dc.description.abstractWe developed a pyrazinamidase gene DNA-sequencing method to rapidly identify pyrazinamide resistance-causing mutations in GenoLyse-treated, smear-positive sputum specimens. The sensitivity and specificity were 90.9 and 100%, respectively, compared to those of MGIT drug susceptibility testing, after the exclusion of synonymous mutations and nonsynonymous mutations previously associated with susceptibility to pyrazinamide.en_ZA
dc.description.urihttp://jcm.asm.org/content/52/11/4056.full
dc.description.versionPublisher's version
dc.format.extent2 pages
dc.identifier.citationStreicher, E. M., et al. 2014. Rapid sequencing of the mycobacterium tuberculosis pncA gene for detection of pyrazinamide susceptibility. Journal of Clinical Microbiology, 52(11): 4056-4057. doi: 10.1128/JCM.02438-14.
dc.identifier.issn0095-1137 (online)
dc.identifier.issn1098-660X (print)
dc.identifier.otherdoi: 10.1128/JCM.02438-14
dc.identifier.urihttp://hdl.handle.net/10019.1/99042
dc.language.isoen_ZAen_ZA
dc.publisherAmerican Society for Microbiology
dc.rights.holderAmerican Society for Microbiology
dc.subjectMycobacterium tuberculosis -- Immunological aspectsen_ZA
dc.subjectPyrazinamide susceptibilityen_ZA
dc.subjectDrug resistanceen_ZA
dc.subjectSequencingen_ZA
dc.titleRapid sequencing of the mycobacterium tuberculosis pncA gene for detection of pyrazinamide susceptibilityen_ZA
dc.typeArticleen_ZA
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