Masters Degrees (Biochemistry)

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https://scholar.sun.ac.za/handle/10019.1/3910

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Browsing Masters Degrees (Biochemistry) by browse.metadata.advisor "Graz, Michael"

Now showing 1 - 2 of 2
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    Laboratory optimization of a protease extraction and purification process from bovine pancreas in preparation for industrial scale up
    (Stellenbosch : Stellenbosch University, 2012-12) De Wet, Tinus Andre; Swart, Pieter; Graz, Michael; Stellenbosch University. Faculty of Science. Dept. of Biochemistry.
    ENGLISH ABSTRACT: This study describes: a) Characterization of traditional methodologies and testing methods used to purify and quantify trypsin and α-chymotrypsin b) Re-engineering / development of a new method for purifying trypsin and α-chymotrypsin that delivered higher product yields and improved control exercised over the process by investigating: i. Extraction methods ii. Centrifugation iii. Ultrafiltration iv. Chymotrypsinogen and trypsin crystallization v. Column chromatography vi. Investigation into different raw material sources for pancreatic enzyme production c) Development of kinetic and ELISA testing methodologies for in-process QC analysis.
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    The optimization of the extraction and purification of horseradish peroxidase from horseradish roots
    (Stellenbosch : Stellenbosch University, 2012-12) Barnard, Almero; Swart, Pieter; Graz, Michael; Stellenbosch University. Faculty of Science. Dept. of Biochemistry.
    ENGLISH ABSTRACT: This study describes: a) the optimization of the current industrial-scale extraction and purification of Horseradish peroxidase from horseradish at BBI Enzymes, focussing on: a. Raw material quality, b. Extraction, c. Ultra-filtration, d. Salt fractionation, e. Diafiltration, f. Ion Exchange Chromatography, b) developing an new in-process microtitre plate calorimetric assay, c) characterization of main groups of HRP relevant to BBI Enzymes by SDS-PAGE- and HPLC analysis.