The optimization of the extraction and purification of horseradish peroxidase from horseradish roots
Date
2012-12
Authors
Barnard, Almero
Journal Title
Journal ISSN
Volume Title
Publisher
Stellenbosch : Stellenbosch University
Abstract
ENGLISH ABSTRACT: This study describes:
a) the optimization of the current industrial-scale extraction and purification of Horseradish
peroxidase from horseradish at BBI Enzymes, focussing on:
a. Raw material quality,
b. Extraction,
c. Ultra-filtration,
d. Salt fractionation,
e. Diafiltration,
f. Ion Exchange Chromatography,
b) developing an new in-process microtitre plate calorimetric assay,
c) characterization of main groups of HRP relevant to BBI Enzymes by SDS-PAGE- and HPLC
analysis.
AFRIKAANSE OPSOMMING: Hierdie studie beskryf: a) die optimisering van die huidige industriële-skaal ekstraksie en suiwering van peperwortelperoksidase vanuit peperwortel by BBI Enzymes, deur te focus op: a. Rou material kwaliteit, b. Ekstraksie, c. Ultra-filtrasie, d. Sout fraksionering, e. Diafiltrasie, f. Ioon-uitruilchromatografie b) Ontwikkeling van ‘n nuwe in-proses mikro-titer gebaseerde kalorimetriese toetsmetode c) die karakterisering van die hoof groepe peperwortel-peroksidase belangrik vir BBI Enzymes.
AFRIKAANSE OPSOMMING: Hierdie studie beskryf: a) die optimisering van die huidige industriële-skaal ekstraksie en suiwering van peperwortelperoksidase vanuit peperwortel by BBI Enzymes, deur te focus op: a. Rou material kwaliteit, b. Ekstraksie, c. Ultra-filtrasie, d. Sout fraksionering, e. Diafiltrasie, f. Ioon-uitruilchromatografie b) Ontwikkeling van ‘n nuwe in-proses mikro-titer gebaseerde kalorimetriese toetsmetode c) die karakterisering van die hoof groepe peperwortel-peroksidase belangrik vir BBI Enzymes.
Description
Thesis (MSc)--Stellenbosch University, 2012.
Keywords
Horseradish peroxidase, Enzymes -- Purification, Dissertations -- Biochemistry, Theses -- Biochemistry