The influence of dual CYP17 expression on adrenal steroidogenesis in the South African Angora Goat

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dc.contributor.advisorSwart, P.en_ZA
dc.contributor.advisorSwart, Amanda C.en_ZA
dc.contributor.authorStorbeck, Karl-Heinzen_ZA
dc.contributor.otherStellenbosch University. Faculty of Science. Dept. of Biochemistry.en_ZA
dc.date.accessioned2008-11-17T12:08:09Zen_ZA
dc.date.accessioned2010-06-01T08:12:27Z
dc.date.available2008-11-17T12:08:09Zen_ZA
dc.date.available2010-06-01T08:12:27Z
dc.date.issued2008-12
dc.descriptionThesis (PhD (Biochemistry))--Stellenbosch University, 2008.
dc.description.abstractThis study describes: • the cloning and sequencing of cytochrome P450 17 -hydroxylase/17,20 lyase (CYP17), 3 -hydroxysteroid dehydrogenase (3 HSD) and cytochrome b5 from the South African Angora goat; • the identification of two CYP17 genes encoding two unique CYP17 isoforms in the Angora goat; • the development of a UPLC-APCI-LC method for the separation and quantification of seven adrenal steroids; • the characterisation of the enzymatic activity of the two Angora CYP17 isoforms expressed in non-steroidogenic COS-1 cells. The Km and Vvalues for the metabolism of pregnenolone and progesterone were determined; • the development of a rapid and accurate real-time PCR genotyping test for CYP17 in Angora goats. Three unique genotypes were identified; • the determination of blood cortisol levels upon the stimulation of the HPAaxis by intravenous insulin injection in the three Angora goat genotypes.en
dc.identifier.urihttp://hdl.handle.net/10019.1/1101
dc.language.isoen
dc.publisherStellenbosch : Stellenbosch University
dc.rights.holderStellenbosch University
dc.subjectCytochrome P450en
dc.subjectCYP17 expressionen_ZA
dc.subjectHydrocortisoneen_ZA
dc.subjectAngora goats -- Physiologyen_ZA
dc.subjectAdrenocortical hormonesen_ZA
dc.subjectDissertations -- Biochemistryen_ZA
dc.subjectTheses -- Biochemistryen_ZA
dc.titleThe influence of dual CYP17 expression on adrenal steroidogenesis in the South African Angora Goaten_ZA
dc.typeThesisen_ZA
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