The influence of dual CYP17 expression on adrenal steroidogenesis in the South African Angora Goat
Date
2008-12
Authors
Journal Title
Journal ISSN
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Publisher
Stellenbosch : Stellenbosch University
Abstract
This study describes:
• the cloning and sequencing of cytochrome P450 17 -hydroxylase/17,20
lyase (CYP17), 3 -hydroxysteroid dehydrogenase (3 HSD) and
cytochrome b5 from the South African Angora goat;
• the identification of two CYP17 genes encoding two unique CYP17
isoforms in the Angora goat;
• the development of a UPLC-APCI-LC method for the separation and
quantification of seven adrenal steroids;
• the characterisation of the enzymatic activity of the two Angora CYP17
isoforms expressed in non-steroidogenic COS-1 cells. The Km and Vvalues
for the metabolism of pregnenolone and progesterone were
determined;
• the development of a rapid and accurate real-time PCR genotyping test for
CYP17 in Angora goats. Three unique genotypes were identified;
• the determination of blood cortisol levels upon the stimulation of the HPAaxis
by intravenous insulin injection in the three Angora goat genotypes.
Description
Thesis (PhD (Biochemistry))--Stellenbosch University, 2008.
Keywords
Cytochrome P450, CYP17 expression, Hydrocortisone, Angora goats -- Physiology, Adrenocortical hormones, Dissertations -- Biochemistry, Theses -- Biochemistry