Aspects of the pharmacokinetics of hypoxoside in rodents

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dc.contributor.authorBester, Christoffel Carolusen_ZA
dc.contributor.otherStellenbosch University. Faculty of Medicine and Health Sciences. Dept. of Medical Microbiology.en_ZA
dc.date.accessioned2012-08-27T11:38:42Z
dc.date.available2012-08-27T11:38:42Z
dc.date.issued1993
dc.descriptionThesis (M. Sc.(Med.)) -- University of Stellenbosch, 1993.
dc.description.abstractENGLISH ABSTRACT: Certain aspects of the pharmacokinetics of a novel diglucoside, hypoxoside, were investigated in mice and rats. After intragastric dosage of the mouse, hypoxoside was hydrolysed to its aglycone, rooperol. Most enzyme activity occurred in the mouse caecum with intestinal bacteria playing a major role in producing the hydrolytic enzymes. Caecalase extracts hydrolysed hypoxoside in vitro but this activity ceased after the mice were treated with antibiotics, suggesting bacteria as the enzyme source. However, in vivo tests with similar antibiotic-treated mice seemed to demonstrate a minor second, possibly mucosal source of hypoxoside hydrolysing B-glucosidase. An attempt to isolate a single strain of faecal bacteria capable of this activity was unsuccessful although hypoxoside was hydrolysed by pure cultures of some non-intestinal clinical isolatesen_ZA
dc.description.abstractAFRIKAANSE OPSOMMING: Sekere aspekte van die farmakokinetika van 'n nuwe diglukosied, hipoksosied, was ondersoek deur gebruik te maak van muise en rotte. Na intragastriese dosering van muise word hipoksosied na sy aglikon, rooperol gehidroliseer. Die meeste ensiemaktiwiteit he tin die muissekum plaasgevind waar intestinale bakterieë 'n vername rol speel in die produksie van die hidrolitiese ensieme. Sekalase ekstrakte het hipoksosied in vitro gehidroliseer maar hierdie proses het ten einde gekom na behandeling van die muise met antibiotika. Dit wil dus voorkom asof bakterieë die bron van die ensiem is. In vivo toetse met soortgelyke behandelde muise, dui op die bestaan van 'n tweede, moontlik mukosale, bron van die ensiem. 'n Poging om 'n reinkultuur van fekale bakterië te isoleer wat in staat was om hipoksosied te hidroliseer was onsuksesvol, alhoewel reinkulture van sekere nie-intestinale kliniese isolate dit wel kon doen.af_ZA
dc.format.extent159 pages
dc.identifier.urihttp://hdl.handle.net/10019.1/57866
dc.language.isoen_ZA
dc.publisherStellenbosch : Stellenbosch University
dc.rights.holderStellenbosch University
dc.subject.lcshPharmacokineticsen_ZA
dc.subject.lcshRodents as laboratory animalsen_ZA
dc.subject.lcshDissertations -- Medicineen_ZA
dc.titleAspects of the pharmacokinetics of hypoxoside in rodentsen_ZA
dc.typeThesis
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