Antioxidant activities of Basella alba aqueous leave extract in blood, pancreas, and gonadal tissues of diabetic male Wistar rats
Date
2018
Journal Title
Journal ISSN
Volume Title
Publisher
Wolters Kluwer
Abstract
Background: Oxidative stress is frequently identified as a key element
in the pathophysiology of many complications of diabetes mellitus,
including reproductive complications. The antioxidant potential of
medicinal plants have been suggested for therapeutic focus of diseases
in recent reports. Objective: To investigate the effect of Basella
alba (Ba) aqueous leave extract on diabetes‑induced oxidative stress.
Materials and Methods: Forty male Wistar rats (8–10 weeks) were
randomly divided into four groups (n = 10) and treated as follows;
Control (C + Ns) and Diabetic (D + Ns) animals received oral normal saline
0.5 ml/100 g body weight daily, while Healthy Treatment (H + Ba) and
Diabetic Treatment (D + Ba) rats were given Ba extract at an oral dose
of 200 mg/kg body weight daily. Treatment was by gavage and lasted
4 weeks in all groups. Diabetes was induced in D + Ns and D + Ba rats
by single intraperitoneal injection of streptozotocin (55 mg/kg) and fasting
blood sugar (FBS) recorded weekly in all rats afterwards. Animals were
euthanized at the end of the experiment and blood samples, pancreas,
testes, and epididymis were preserved for analysis of oxidative stress
biomarkers. Results: Oral administration of aqueous leave extract of
Ba significantly (P < 0.0001) lowered FBS in D + Ba rats. There was
significantly higher blood superoxide dismutase activity and serum ferric
reducing antioxidant power, but lower serum concentration of conjugated
dienes and thiobarbituric acid reactive substances in D + Ba compared to
D + Ns rats (P < 0.05). Conclusion: Ba exerts antioxidant effects in the
gonads by enhancing antioxidant parameters in circulating blood, but not
necessarily in the gonadal tissues.
Description
CITATION: Arokoyo, D. S. et al. 2018. Antioxidant activities of Basella alba aqueous leave extract in blood, pancreas, and gonadal tissues of diabetic male Wistar rats. Pharmacognosy Research, 10:31-6, doi:10.4103/pr.pr_84_17.
The original publication is available at https://www.phcogres.com
The original publication is available at https://www.phcogres.com
Keywords
Basella alba, Antioxidants
Citation
Arokoyo, D. S. et al. 2018. Antioxidant activities of Basella alba aqueous leave extract in blood, pancreas, and gonadal tissues of diabetic male Wistar rats. Pharmacognosy Research, 10:31-6, doi:10.4103/pr.pr_84_17.