Partial characterization of a bacterial acyltransferase enzyme for potential application in dairy processing
| dc.contributor.advisor | Swart, Pieter | en_ZA |
| dc.contributor.author | Hayward, Stefan | en_ZA |
| dc.contributor.other | Stellenbosch University. Faculty of Science. Dept. of Biochemistry. | en_ZA |
| dc.date.accessioned | 2014-04-16T17:30:49Z | |
| dc.date.available | 2014-04-16T17:30:49Z | |
| dc.date.issued | 2014-04 | |
| dc.description | Thesis (MSc)--Stellenbosch University, 2014. | en_ZA |
| dc.description.abstract | ENGLISH ABSTRACT: This study describes: the evaluation of the current, and potential assay methods for the quantification of cholesterol, cholesteryl esters and free fatty acids in milk and the application thereof ; an account of the difficulties associated with the usage of FoodPro® Cleanline, an enzyme preparation used as processing aid, during ultra-high temperature processing of milk ; the development of activity assays which can be used for the kinetic characterization of glycerophospholipid cholesterol acyltransferase, the active enzyme in FoodPro® Cleanline ; the development of an accurate and facile activity assay, and the validation thereof, which can be used for the validation of enzyme activity prior to dosage of milk with FoodPro® Cleanline. | en_ZA |
| dc.description.abstract | AFRIKAANSE OPSOMMING: Hierdie studie beskryf: die evaluering van die huidige, en potensiële, metodes vir die kwantifisering van cholesterol, cholesteriel esters en vryvetsure in melk, sowel as die toepassing van hieridie metodes ; 'n verduideliking van die moeilikhede wat ondervind word gedurende die gebruik van FoodPro® Cleanline, 'n ensiempreparaat vir gebruik as 'n verwerkingshulpmiddel, tydens ultrahoë-temperatuurprosessering van melk ; die ontwikkeling van aktiwiteitsbepalings metodes vir gebruik in kinetiese karakterisering van gliserofosfolipied cholesterol asieltransferase, die aktiewe ensiem in FoodPro® Cleanline ; die ontwikkeling van 'n akkurate, eenvoudige aktiwiteitsbepalings metode, en bevestiging van hierdie metode, wat gebruik kan word vir kwalitieitskontrole alvorens die dosering van melk met FoodPro® Cleanline. | af_ZA |
| dc.format.extent | xv, 118 p. : ill. | |
| dc.identifier.uri | http://hdl.handle.net/10019.1/86636 | |
| dc.language.iso | en_ZA | en_ZA |
| dc.publisher | Stellenbosch : Stellenbosch University | en_ZA |
| dc.rights.holder | Stellenbosch University | |
| dc.subject | Milk -- Microbiology | en_ZA |
| dc.subject | Fouling | en_ZA |
| dc.subject | Dairy processing | en_ZA |
| dc.subject | Dissertations -- Biochemistry | en_ZA |
| dc.subject | Theses -- Biochemistry | en_ZA |
| dc.subject | UCTD | en_ZA |
| dc.title | Partial characterization of a bacterial acyltransferase enzyme for potential application in dairy processing | en_ZA |
| dc.type | Thesis | en_ZA |