dc.contributor.author	Karboul, Anis
dc.contributor.author	Gey van Pittius, Nicolaas C.
dc.contributor.author	Namouchi, Amine
dc.contributor.author	Vincent, Veronique
dc.contributor.author	Sola, Christophe
dc.contributor.author	Rastogi, Nalin
dc.contributor.author	Suffys, Philip
dc.contributor.author	Fabre, Michel
dc.contributor.author	Cataldi, Angel
dc.contributor.author	Huard, Richard C.
dc.contributor.author	Kurepina, Natalia
dc.contributor.author	Kreiswirth, Barry
dc.contributor.author	Ho, John L.
dc.contributor.author	Gutierrez, M. Cristina
dc.contributor.author	Mardassi, Helmi
dc.date.accessioned	2010-12-14T13:26:13Z
dc.date.available	2010-12-14T13:26:13Z
dc.date.issued	2006-12
dc.date.updated	2010-11-09T12:55:21Z
dc.description.abstract	Background: The highly homologous PE_PGRS (Proline-glutamic acid_polymorphic GC-rich repetitive sequence) genes are members of the PE multigene family which is found only in mycobacteria. PE genes are particularly abundant within the genomes of pathogenic mycobacteria where they seem to have expanded as a result of gene duplication events. PE_PGRS genes are characterized by their high GC content and extensive repetitive sequences, making them prone to recombination events and genetic variability. Results: Comparative sequence analysis of Mycobacterium tuberculosis genes PE_PGRS17 (Rv0978c) and PE_PGRS18 (Rv0980c) revealed a striking genetic variation associated with this typical tandem duplicate. In comparison to the M. tuberculosis reference strain H37Rv, the variation (named the 12/40 polymorphism) consists of an in-frame 12-bp insertion invariably accompanied by a set of 40 single nucleotide polymorphisms (SNPs) that occurs either in PE_PGRS17 or in both genes. Sequence analysis of the paralogous genes in a representative set of worldwide distributed tubercle bacilli isolates revealed data which supported previously proposed evolutionary scenarios for the M. tuberculosis complex (MTBC) and confirmed the very ancient origin of "M. canettii" and other smooth tubercle bacilli. Strikingly, the identified polymorphism appears to be coincident with the emergence of the post-bottleneck successful clone from which the MTBC expanded. Furthermore, the findings provide direct and clear evidence for the natural occurrence of gene conversion in mycobacteria, which appears to be restricted to modern M. tuberculosis strains. Conclusion: This study provides a new perspective to explore the molecular events that accompanied the evolution, clonal expansion, and recent diversification of tubercle bacilli.	en_ZA
dc.description.version	Peer Reviewed
dc.identifier.citation	Karboul, A, Gey van Pittius, NC, Namouchi, A, Vincent, V, Sola, C, Rastogi, N, Suffys, P, Fabre, M, Cataldi, A, Huard, RC, Kurepina, N, Kreiswirth, B, Ho, JL, Gutierrez, MC & Mardassi, H 2006, 'Insights into the evolutionary history of tubercle bacilli as disclosed by genetic rearrangements within a PE_PGRS duplicated gene pair', BMC Evolutionary Biology, 6(1):107.	en_ZA
dc.identifier.issn	1471-2148
dc.identifier.other	http://dx.doi.org/10.1186/1471-2148-6-107
dc.identifier.uri	http://hdl.handle.net/10019.1/5117
dc.language.iso	en_ZA	en_ZA
dc.language.rfc3066	en
dc.publisher	BioMed Central	en_ZA
dc.rights.holder	Karboul et al.; licensee BioMed Central Ltd.	en_ZA
dc.subject	PE_PGRS (Proline-glutamic acid_polymorphic GC-rich repetitive sequence) genes	en_ZA
dc.subject	Mycobacterium tuberculosis	en_ZA
dc.subject	Tuberculosis -- Research	en_ZA
dc.subject	Pathogenic bacteria	en_ZA
dc.title	Insights into the evolutionary history of tubercle bacilli as disclosed by genetic rearrangements within a PE_PGRS duplicated gene pair	en_ZA
dc.type	Article	en_ZA

Insights into the evolutionary history of tubercle bacilli as disclosed by genetic rearrangements within a PE_PGRS duplicated gene pair

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