Regulation of endo-polygalacturonase activity in Saccharomyces cerevisiae

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dc.contributor.authorLouw, C.en_ZA
dc.contributor.authorYoung, P. R.en_ZA
dc.contributor.authorVan Rensburg, P.en_ZA
dc.contributor.authorDivol, Benoiten_ZA
dc.date.accessioned2011-05-15T16:03:26Z
dc.date.available2011-05-15T16:03:26Z
dc.date.issued2010
dc.description.abstractPectolytic activity in Saccharomyces cerevisiae is due to the secretion of an endo-polygalacturonase encoded by the PGU1 gene. The ability to degrade polygalacturonic acid has been shown to vary between different strains. In this study, we attempted to elucidate how pectolytic activity is regulated in S. cerevisiae and to determine whether the means of regulation differ between strains. Saccharomyces cerevisiae strains from different genetic backgrounds, with varying ability to degrade pectin, were compared. Activity was found not to be regulated by sequence differences in the PGU1 gene, but by the transcription level of the gene. Expression of PGU1 was found to be determined by the transcription level of its two transcription factors TEC1 and STE12. The activation of PGU1 transcription by galactose was found to be strain specific, independent of the strain being an industrial or a domesticated one. The EUROSCARF yeast deletion library was screened for genes encoding inhibitors and activators of polygalacturonase activity. Fourteen strains were identified, in which deletion of a specific gene resulted in a recovery of polygalacturonase activity; these genes were identified as encoding inhibitors of polygalacturonase activity, and two activators were identified. © 2009 Federation of European Microbiological Societies.
dc.description.versionArticle
dc.identifier.citationFEMS Yeast Research
dc.identifier.citation10
dc.identifier.citation1
dc.identifier.issn15671356
dc.identifier.other10.1111/j.1567-1364.2009.00584.x
dc.identifier.urihttp://hdl.handle.net/10019.1/12620
dc.subjectgalactose
dc.subjectpectin
dc.subjectpolygalacturonase
dc.subjectpolygalacturonic acid
dc.subjecttranscription factor
dc.subjectarticle
dc.subjectdegradation
dc.subjectenzyme activity
dc.subjectenzyme regulation
dc.subjectenzyme release
dc.subjectfungal gene
dc.subjectfungal strain
dc.subjectgene identification
dc.subjectgene sequence
dc.subjectgenetic transcription
dc.subjectnonhuman
dc.subjectPGU1 gene
dc.subjectSaccharomyces cerevisiae
dc.subjectSTE12 gene
dc.subjectTEC1 gene
dc.subjecttranscription initiation
dc.subjectDNA-Binding Proteins
dc.subjectGalactose
dc.subjectGene Expression Regulation, Enzymologic
dc.subjectGene Expression Regulation, Fungal
dc.subjectPectins
dc.subjectPolygalacturonase
dc.subjectSaccharomyces cerevisiae
dc.subjectSaccharomyces cerevisiae Proteins
dc.subjectTranscription Factors
dc.subjectTranscription, Genetic
dc.subjectSaccharomyces cerevisiae
dc.titleRegulation of endo-polygalacturonase activity in Saccharomyces cerevisiae
dc.typeArticle
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