In vitro a-glucosidase inhibition by honeybush (Cyclopia genistoides) food ingredient extract-potential for dose reduction of acarbose through synergism

Simple item page
dc.contributor.authorMiller, Neilen_ZA
dc.contributor.authorMalherbe, Christiaan J.en_ZA
dc.contributor.authorJoubert, Elizabethen_ZA
dc.date.accessioned2022-07-11T06:55:43Z
dc.date.available2022-07-11T06:55:43Z
dc.date.issued2020-06-29
dc.descriptionCITATION: Miller, N.; Malherbe, C. J. & Joubert, E. 2020. In vitro α-glucosidase inhibition by honeybush (Cyclopia genistoides) food ingredient extract—potential for dose reduction of acarbose through synergism. Food and Function, 11:6476–6486. doi:10.1039/d0fo01306den_ZA
dc.descriptionThe original publication is available at http://pubs.rsc.org/en/Journals/Journal/FOen_ZA
dc.description.abstractExtracts of Cyclopia species are used as food ingredients. In vitro α-glucosidase (AG) inhibition by ultrafiltered C. genistoides extract, fractions enriched in xanthones (XEF) and benzophenones (BEF), as well as mangiferin, isomangiferin, 3-β-D-glucopyranosyliriflophenone (I3G) and 3-β-D-glucopyranosyl-4-O-β-D-glucopyranosyliriflophenone (IDG) was determined with acarbose as positive control. XEF was more potent than the extract and BEF (IC50 = 43.3, 95.5 and 205.7 μg mL−1, respectively). Compounds demonstrated potency in the descending order: acarbose (IC50 = 44.3 μM) > mangiferin (102.2 μM) > isomangiferin (119.8 μM) > I3G (237.5 μM) > IDG (299.4 μM). The combination index (CI) was used to determine synergism (CI < 0.7) as demonstrated for combinations of acarbose with XEF, BEF or the respective compounds at 50% and 75% effect levels. The greatest potential acarbose dose reductions (>six-fold) across all effect levels were calculated for combinations of acarbose with mangiferin or isomangiferin, explaining the greater acarbose dose reduction potential of XEF vs. BEF. The effect of batch-to-batch variation (n = 10) of raw plant material on AG inhibition was quantified at a fixed concentration (160 μg mL−1). XEFs (xanthone content = 223–481 g kg−1) achieved AG inhibition of 63–72%, whereas BEFs (benzophenone content = 114–251 g kg−1) achieved AG inhibition of 26–34%, with weak linear correlation (R2 < 0.43) between target compound content of the fractions and their achieved AG inhibition. Thus, extract fractions of C. genistoides, enriched in xanthones and benzophenones, show potential in reducing the effective dose of acarbose required to prevent postprandial hyperglycaemia.en_ZA
dc.description.urihttps://pubs.rsc.org/en/content/articlelanding/2020/fo/d0fo01306d
dc.description.versionPublishers versionen_ZA
dc.format.extent11 pages : illustrationsen_ZA
dc.identifier.citationMiller, N.; Malherbe, C. J. & Joubert, E. 2020. In vitro α-glucosidase inhibition by honeybush (Cyclopia genistoides) food ingredient extract—potential for dose reduction of acarbose through synergism. Food and Function, 11:6476–6486. doi:10.1039/d0fo01306den_ZA
dc.identifier.issn2042-650X (online)
dc.identifier.issn2042-6496 (print)
dc.identifier.otherdoi:10.1039/d0fo01306d
dc.identifier.urihttp://hdl.handle.net/10019.1/125481
dc.language.isoen_ZAen_ZA
dc.publisherRoyal Society of Chemistryen_ZA
dc.rights.holderThe Royal Society of Chemistryen_ZA
dc.subjectIn vitro α-glucosidaseen_ZA
dc.subjectCyclopia genistoides extracten_ZA
dc.subjectHoneybush (Cyclopia genistoides)en_ZA
dc.subjectDose reduction of acarboseen_ZA
dc.subjectDrug synergismen_ZA
dc.titleIn vitro a-glucosidase inhibition by honeybush (Cyclopia genistoides) food ingredient extract-potential for dose reduction of acarbose through synergismen_ZA
dc.typeArticleen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
miller_invitro_2020.pdf
Size:
1.17 MB
Format:
Adobe Portable Document Format
Description:
Download article
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Research Articles (Food Science)