Evaluation of a nisin-eluting nanofiber scaffold to treat Staphylococcus aureus-induced skin infections in mice

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dc.contributor.authorHeunis, Tiaan D. J.en_ZA
dc.contributor.authorSmith, Carineen_ZA
dc.contributor.authorDicks, Leon Milner Theodore, 1961-en_ZA
dc.date.accessioned2014-03-03T07:59:54Z
dc.date.available2014-03-03T07:59:54Z
dc.date.issued2013-08
dc.descriptionHeunis, T. D. J., Smith, C. & Dicks, L. M. T. 2013. Evaluation of a nisin-eluting nanofiber scaffold to treat Staphylococcus aureus-induced skin infections in mice. Antimicrobial Agents and Chemotherapy, 57(8):3928-3935, doi:10.1128/AAC.00622-13, http://aac.asm.org/en_ZA
dc.descriptionThe original publication is available at https://aac.asm.orgen_ZA
dc.description.abstractStaphylococcus aureus is a virulent pathogen and a major causative agent of superficial and invasive skin and soft tissue infections (SSSTIs). Antibiotic resistance in S. aureus, among other bacterial pathogens, has rapidly increased, and this is placing an enormous burden on the health care sector and has serious implications for infected individuals, especially immunocompromised patients. Alternative treatments thus need to be explored to continue to successfully treat infections caused by S. aureus, including antibiotic-resistant strains of S. aureus. In this study, an antimicrobial nanofiber wound dressing was generated by electrospinning nisin (Nisaplin) into poly(ethylene oxide) and poly(d,l-lactide) (50:50) blend nanofibers. Active nisin diffused from the nanofiber wound dressings for at least 4 days in vitro, as shown by consecutive transfers onto plates seeded with strains of methicillin-resistant S. aureus (MRSA). The nisin-containing nanofiber wound dressings significantly reduced S. aureus Xen 36 bioluminescence in vivo and viable cell numbers in a murine excisional skin infection model. The bacterial burden of wounds treated with nisin-containing nanofiber wound dressings was 4.3 × 102 CFU/wound, whereas wounds treated with control nanofiber wound dressings had 2.2 × 107 CFU/wound on the last day of the trial (day 7). Furthermore, the wound dressings stimulated wound closure of excisional wounds, and no adverse effects were observed by histological analysis. Nisin-containing nanofiber wound dressings have the potential to treat S. aureus skin infections and to potentially accelerate wound healing of excisional wounds.en_ZA
dc.description.versionPost-printen_ZA
dc.format.extent17 p. : ill.
dc.identifier.citationHeunis, T. D. J., Smith, C. & Dicks, L. M. T. 2013. Evaluation of a nisin-eluting nanofiber scaffold to treat Staphylococcus aureus-induced skin infections in mice. Antimicrobial Agents and Chemotherapy, 57(8):3928-3935, doi:10.1128/AAC.00622-13.en_ZA
dc.identifier.issn1098-6596 (online)
dc.identifier.issn0066-4804 (print)
dc.identifier.otherdoi:10.1128/AAC.00622-13
dc.identifier.urihttp://hdl.handle.net/10019.1/86154
dc.language.isoen_ZAen_ZA
dc.publisherAmerican Society for Microbiologyen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectStaphylococcus aureusen_ZA
dc.subjectSkin infectionen_ZA
dc.subjectMice as test subjectsen_ZA
dc.subjectSuperficial and invasive Skin and Soft Tissue Infections (SSSTIs)en_ZA
dc.titleEvaluation of a nisin-eluting nanofiber scaffold to treat Staphylococcus aureus-induced skin infections in miceen_ZA
dc.typeArticleen_ZA
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