Proteomics and transcriptomics of the BABA-induced resistance response in potato using a novel functional annotation approach

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dc.contributor.authorBengtsson, Therese
dc.contributor.authorWeighill, Deborah
dc.contributor.authorProux-Wera, Estelle
dc.contributor.authorLevander, Fredrik
dc.contributor.authorResjo, Svante
dc.contributor.authorBurra, Dharani Dhar
dc.contributor.authorMoushib, Laith Ibrahim
dc.contributor.authorHedley, Pete E.
dc.contributor.authorLiljeroth, Erland
dc.contributor.authorJacobson, Dan
dc.contributor.authorAlexandersson, Erik
dc.contributor.authorAndreasson, Erik
dc.date.accessioned2014-05-29T09:11:20Z
dc.date.available2014-05-29T09:11:20Z
dc.date.issued2014-04
dc.date.updated2014-05-02T11:12:37Z
dc.descriptionPlease site as follows:en_ZA
dc.descriptionBengtsson, T. et al. 2014. Proteomics and transcriptomics of the BABA-induced resistance response in potato using a novel functional annotation approach. BMC Genomics, 15(1):315, doi:10.1186/1471-2164-15-315.en_ZA
dc.descriptionThe original publication is available at http://www.biomedcentral.com/1471-2164/15/315en_ZA
dc.description.abstractAbstract Background Induced resistance (IR) can be part of a sustainable plant protection strategy against important plant diseases. β-aminobutyric acid (BABA) can induce resistance in a wide range of plants against several types of pathogens, including potato infected with Phytophthora infestans. However, the molecular mechanisms behind this are unclear and seem to be dependent on the system studied. To elucidate the defence responses activated by BABA in potato, a genome-wide transcript microarray analysis in combination with label-free quantitative proteomics analysis of the apoplast secretome were performed two days after treatment of the leaf canopy with BABA at two concentrations, 1 and 10 mM. Results Over 5000 transcripts were differentially expressed and over 90 secretome proteins changed in abundance indicating a massive activation of defence mechanisms with 10 mM BABA, the concentration effective against late blight disease. To aid analysis, we present a more comprehensive functional annotation of the microarray probes and gene models by retrieving information from orthologous gene families across 26 sequenced plant genomes. The new annotation provided GO terms to 8616 previously un-annotated probes. Conclusions BABA at 10 mM affected several processes related to plant hormones and amino acid metabolism. A major accumulation of PR proteins was also evident, and in the mevalonate pathway, genes involved in sterol biosynthesis were down-regulated, whereas several enzymes involved in the sesquiterpene phytoalexin biosynthesis were up-regulated. Interestingly, abscisic acid (ABA) responsive genes were not as clearly regulated by BABA in potato as previously reported in Arabidopsis. Together these findings provide candidates and markers for improved resistance in potato, one of the most important crops in the world.en_ZA
dc.description.versionPublishers' Versionen_ZA
dc.format.extent19 p. : ill.
dc.identifier.citationBengtsson, T. et al. 2014. Proteomics and transcriptomics of the BABA-induced resistance response in potato using a novel functional annotation approach. BMC Genomics, 15(1):315, doi:10.1186/1471-2164-15-315.en_ZA
dc.identifier.issn1471-2164 (online)
dc.identifier.otherdoi:10.1186/1471-2164-15-315
dc.identifier.urihttp://hdl.handle.net/10019.1/86853
dc.language.isoen_ZAen_ZA
dc.publisherBioMed Centralen_ZA
dc.rights.holderTherese Bengtsson et al.; licensee BioMed Central Ltd.en_ZA
dc.subjectPlant protectionen_ZA
dc.subjectPotatoes -- Diseases and pests resistanceen_ZA
dc.subjectPhytophthora infestansen_ZA
dc.titleProteomics and transcriptomics of the BABA-induced resistance response in potato using a novel functional annotation approachen_ZA
dc.typeArticleen_ZA
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