Differential modulation of gene expression encoding hepatic and renal xenobiotic metabolizing enzymes by an aspalathin-enriched rooibos extract and aspalathin

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dc.contributor.authorAbrahams, Sameegaen_ZA
dc.contributor.authorSamodien, Sedickaen_ZA
dc.contributor.authorLilly, Mariskaen_ZA
dc.contributor.authorJoubert, Elizabethen_ZA
dc.contributor.authorGelderblom, Wentzelen_ZA
dc.date.accessioned2021-10-26T12:33:16Z
dc.date.available2021-10-26T12:33:16Z
dc.date.issued2019
dc.descriptionCITATION: Abrahams, S. et al. 2019. Differential Modulation of Gene Expression Encoding Hepatic and Renal Xenobiotic Metabolizing Enzymes by an AspalathinEnriched Rooibos Extract and Aspalathin. Planta Med, 85(1):6–13. doi:10.1055/a-0656-7500
dc.descriptionThe original publication is available at https://www.thieme.com/books-main/biochemistry/product/3494-planta-medica
dc.description.abstractModulation of the expression of hepatic and renal genes encoding xenobiotic metabolizing enzymes by an aspalathin-enriched green rooibos (Aspalathus linearis) extract (GRE) was investigated in the liver and kidneys of F344 rats following dietary exposure of 28 d, as well as selected xenobiotic metabolizing genes in rat primary hepatocytes. In the liver, GRE upregulated genes (p < 0.05) encoding aldehyde dehydrogenase, glucose phosphate isomerase, and cytochrome P450 while 17β-hydroxysteroid dehydrogenase 2 (Hsd17β2) was downregulated. In primary hepatocytes, GRE lacked any effect, while aspalathin downregulated Hsd17β2, mimicking the effect of GRE in vivo, and upregulated catechol-O-methyl transferase and marginally (p < 0.1) cytochrome P450 2e1. In the kidneys, GRE upregulated (p < 0.05) genes encoding the phase II xenobiotic metabolism enzymes, glutathione-S-transferase mµ and microsomal glutathione-S-transferase, while downregulating genes encoding the ATP binding cassette transporter, cytochrome P450, gamma glutamyltransferase 1, and N-acetyltransferase 1. Differential modulation of the expression of xenobiotic metabolizing genes in vivo and in vitro by GRE is dose-related, duration of exposure, the tissue type, and interactions between specific polyphenol and/or combinations thereof. Aspalathin is likely to be responsible for the downregulation of estradiol and testosterone catabolism by GRE in the liver. The differential gene expression by GRE in the liver and kidneys could, depending on the duration exposure and dose utilized, determine the safe use of such an extract in humans for specific health and/or disease outcomes.en_ZA
dc.description.urihttps://www.thieme-connect.de/products/ejournals/abstract/10.1055/a-0656-7500
dc.description.versionPublisher's version
dc.format.extent8 pages
dc.identifier.citationAbrahams, S. et al. 2019. Differential Modulation of Gene Expression Encoding Hepatic and Renal Xenobiotic Metabolizing Enzymes by an AspalathinEnriched Rooibos Extract and Aspalathin. Planta Med, 85(1):6–13. doi:10.1055/a-0656-7500
dc.identifier.issn1439-0221 (online)
dc.identifier.issn0032-0943 (print)
dc.identifier.otherdoi:10.1055/a-0656-7500
dc.identifier.urihttp://hdl.handle.net/10019.1/123314
dc.language.isoen_ZAen_ZA
dc.publisherThieme Gruppe
dc.rights.holderGeorg Thieme Verlag KG Stuttgart
dc.subjectLeguminosaeen_ZA
dc.subjectAspalathus linearisen_ZA
dc.subjectXenobiotic metabolizing enzymesen_ZA
dc.subjectHepatocytesen_ZA
dc.subjectqRT-PCRen_ZA
dc.subjectEnzymes -- Genetic aspectsen_ZA
dc.subjectMicroorganisms -- Effect of xenobiotics onen_ZA
dc.titleDifferential modulation of gene expression encoding hepatic and renal xenobiotic metabolizing enzymes by an aspalathin-enriched rooibos extract and aspalathinen_ZA
dc.typeArticleen_ZA
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