FLO gene-dependent phenotypes in industrial wine yeast strains

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dc.contributor.authorGovender, P.en_ZA
dc.contributor.authorBester, M.en_ZA
dc.contributor.authorBauer, Florianen_ZA
dc.date.accessioned2011-05-15T15:56:35Z
dc.date.available2011-05-15T15:56:35Z
dc.date.issued2010
dc.description.abstractMost commercial yeast strains are nonflocculent. However, controlled flocculation phenotypes could provide significant benefits to many fermentation-based industries. In nonflocculent laboratory strains, it has been demonstratedthat it is possible to adjust flocculation and adhesion phenotypes to desired specifications by altering expression of the otherwise silent but dominant flocculation (FLO) genes. However, FLO genes are characterized by high allele heterogeneity and are subjected to epigenetic regulation. Extrapolation of data obtained in laboratory strains to industrial strains may therefore not always be applicable. Here, we assess the adhesion phenotypes that are associated with the expression of a chromosomal copy of the FLO1, FLO5, or FLO11 open reading frame in two nonflocculent commercial wine yeast strains, BM45 and VIN13. The chromosomal promoters of these genes were replaced with stationary phase-inducible promoters of the HSP30 and ADH2 genes. Under standard laboratory and wine making conditions, the strategy resulted in expected and stable expression patterns of these genes in both strains. However, the specific impact of the expression of individual FLO genes showed significant differences between the two wine strains and with corresponding phenotypes in laboratory strains. The data suggest that optimization of the flocculation pattern of individual commercial strains will have to be based on a strain-by-strain approach. © 2009 Springer-Verlag.
dc.description.versionArticle
dc.identifier.citationApplied Microbiology and Biotechnology
dc.identifier.citation86
dc.identifier.citation3
dc.identifier.issn1757598
dc.identifier.other10.1007/s00253-009-2381-1
dc.identifier.urihttp://hdl.handle.net/10019.1/9934
dc.subjectCell wall proteins
dc.subjectEpigenetic regulation
dc.subjectFLO gene expression
dc.subjectInducible promoter
dc.subjectIndustrial strain
dc.subjectOpen reading frame
dc.subjectStable expression
dc.subjectStationary phase
dc.subjectWine making
dc.subjectWine strains
dc.subjectWine yeast
dc.subjectYeast strain
dc.subjectAdhesion
dc.subjectApproximation theory
dc.subjectFermentation
dc.subjectFlocculation
dc.subjectLaboratories
dc.subjectProteins
dc.subjectWine
dc.subjectYeast
dc.subjectGene expression
dc.subjectglucose
dc.subjectadhesion
dc.subjectallele
dc.subjectbiotechnology
dc.subjectfermentation
dc.subjectflocculation
dc.subjectgene expression
dc.subjectindustrial technology
dc.subjectphenotype
dc.subjectyeast
dc.subjectarticle
dc.subjectchromosome
dc.subjectcontrolled study
dc.subjectfermentation
dc.subjectfungal strain
dc.subjectgene expression
dc.subjectglucose assay
dc.subjectnonhuman
dc.subjectopen reading frame
dc.subjectphenotype
dc.subjectpromoter region
dc.subjectwine
dc.subjectyeast
dc.subjectCell Adhesion
dc.subjectDNA, Fungal
dc.subjectFermentation
dc.subjectIndustrial Microbiology
dc.subjectLectins
dc.subjectMannose-Binding Lectins
dc.subjectMembrane Glycoproteins
dc.subjectMolecular Sequence Data
dc.subjectPromoter Regions, Genetic
dc.subjectSaccharomyces cerevisiae
dc.subjectSaccharomyces cerevisiae Proteins
dc.subjectSequence Analysis, DNA
dc.subjectWine
dc.titleFLO gene-dependent phenotypes in industrial wine yeast strains
dc.typeArticle
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