Africa-wide evaluation of host biomarkers in QuantiFERON supernatants for the diagnosis of pulmonary tuberculosis

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dc.contributor.authorChegou, Novel N.en_ZA
dc.contributor.authorSutherland, Jayne S.en_ZA
dc.contributor.authorNamuganga, Anna-Ritahen_ZA
dc.contributor.authorCorstjens, Paul L. A. M.en_ZA
dc.contributor.authorGeluk, Annemiekeen_ZA
dc.contributor.authorGebremichael, Gebremedhinen_ZA
dc.contributor.authorMendy, Josephen_ZA
dc.contributor.authorMalherbe, Stephanusen_ZA
dc.contributor.authorStanley, Kimen_ZA
dc.contributor.authorVan Der Spuy, Gian D.en_ZA
dc.contributor.authorKriel, Magdalenaen_ZA
dc.contributor.authorLoxton, Andre G.en_ZA
dc.contributor.authorKriel, Belindaen_ZA
dc.contributor.authorSimukonda, Felanjien_ZA
dc.contributor.authorBekele, Yonasen_ZA
dc.contributor.authorSheehama, Jacob A.en_ZA
dc.contributor.authorNelongo, Josefinaen_ZA
dc.contributor.authorVan Der Vyver, Marietaen_ZA
dc.contributor.authorGebrexabher, Atsbehaen_ZA
dc.contributor.authorHailu, Habteyesen_ZA
dc.contributor.authorEsterhuyse, Maria M.en_ZA
dc.contributor.authorRosenkrands, Idaen_ZA
dc.contributor.authorAagard, Clausen_ZA
dc.contributor.authorKidd, Martinen_ZA
dc.contributor.authorKassa, Destaen_ZA
dc.contributor.authorMihret, Adaneen_ZA
dc.contributor.authorHowe, Rawleighen_ZA
dc.contributor.authorCliff, Jacqueline M.en_ZA
dc.contributor.authorCrampin, Amelia C.en_ZA
dc.contributor.authorMayanja-Kizza, Harrieten_ZA
dc.contributor.authorKaufmann, Stefan H. E.en_ZA
dc.contributor.authorDockrell, Hazel M.en_ZA
dc.contributor.authorOttenhoff, Tom H. M.en_ZA
dc.contributor.authorWalzl, Gerharden_ZA
dc.contributor.authorAE-TBC consortiumen_ZA
dc.date.accessioned2020-03-16T09:11:07Z
dc.date.available2020-03-16T09:11:07Z
dc.date.issued2018-02-08
dc.descriptionCITATION: Chegou, N. N., et al. 2018. Africa-wide evaluation of host biomarkers in QuantiFERON supernatants for the diagnosis of pulmonary tuberculosis. Scientific Reports, 8:2675, doi:10.1038/s41598-018-20855-7.
dc.descriptionThe original publication is available at https://www.nature.com
dc.description.abstractWe investigated host-derived biomarkers that were previously identified in QuantiFERON supernatants, in a large pan-African study. We recruited individuals presenting with symptoms of pulmonary TB at seven peripheral healthcare facilities in six African countries, prior to assessment for TB disease. We then evaluated the concentrations of 12 biomarkers in stored QuantiFERON supernatants using the Luminex platform. Based on laboratory, clinical and radiological findings and a pre-established algorithm, participants were classified as TB disease or other respiratory diseases(ORD). Of the 514 individuals included in the study, 179(34.8%) had TB disease, 274(51.5%) had ORD and 61(11.5%) had an uncertain diagnosis. A biosignature comprising unstimulated IFN-γ, MIP-1β, TGF-α and antigen-specific levels of TGF-α and VEGF, identified on a training sample set (n = 311), validated by diagnosing TB disease in the test set (n = 134) with an AUC of 0.81(95% CI, 0.76–0.86), corresponding to a sensitivity of 64.2%(95% CI, 49.7–76.5%) and specificity of 82.7%(95% CI, 72.4–89.9%). Host biomarkers detected in QuantiFERON supernatants can contribute to the diagnosis of active TB disease amongst people presenting with symptoms requiring investigation for TB disease, regardless of HIV status or ethnicity in Africa.en_ZA
dc.description.urihttps://www.nature.com/articles/s41598-018-20855-7
dc.description.versionPublisher's version
dc.format.extent12 pages ; illustrations
dc.identifier.citationChegou, N. N., et al. 2018. Africa-wide evaluation of host biomarkers in QuantiFERON supernatants for the diagnosis of pulmonary tuberculosis. Scientific Reports, 8:2675, doi:10.1038/s41598-018-20855-7
dc.identifier.issn2045-2322 (online)
dc.identifier.otherdoi:10.1038/s41598-018-20855-7
dc.identifier.urihttp://hdl.handle.net/10019.1/107618
dc.language.isoen_ZAen_ZA
dc.publisherNature Research
dc.rights.holderAuthors retain copyright
dc.subjectTuberculosis -- Diagnosis -- Africaen_ZA
dc.subjectTuberculosis -- Testing -- Africaen_ZA
dc.titleAfrica-wide evaluation of host biomarkers in QuantiFERON supernatants for the diagnosis of pulmonary tuberculosisen_ZA
dc.typeArticleen_ZA
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