National sentinel site surveillance for antimicrobial resistance in Klebsiella pneumoniae isolates in South Africa, 2010-2012

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dc.contributor.authorPerovic, Olgaen_ZA
dc.contributor.authorSingh-Moodley, Ashikaen_ZA
dc.contributor.authorDuse, Adrianoen_ZA
dc.contributor.authorBamford, Colleenen_ZA
dc.contributor.authorElliott, G.en_ZA
dc.contributor.authorSwe-Han, Khine Sween_ZA
dc.contributor.authorKularatne, Ranminien_ZA
dc.contributor.authorLowman, Warrenen_ZA
dc.contributor.authorWhitelaw, Andrewen_ZA
dc.contributor.authorNana, Trushaen_ZA
dc.contributor.authorWadula, Jeanetteen_ZA
dc.contributor.authorLekalakala, Ruthen_ZA
dc.contributor.authorSaif, Adrienneen_ZA
dc.contributor.authorFortuin De-Smit, Melonyen_ZA
dc.contributor.authorMarais, Elseen_ZA
dc.date.accessioned2015-12-10T09:25:51Z
dc.date.available2015-12-10T09:25:51Z
dc.date.issued2014-08
dc.descriptionPlease cite as follows: Perovic, O. et al. 2014. National sentinel site surveillance for antimicrobial resistance in Klebsiella pneumoniae isolates in South Africa, 2010-2012. South African Medical Journal, 104(8):563-568, doi:10.7196/SAMJ.7617.en_ZA
dc.descriptionThe original publication is available at http://www.samj.org.zaen_ZA
dc.description.abstractBackground. The increasing rates of antimicrobial resistance observed in the nosocomial pathogen Klebsiella pneumoniae are of major public health concern worldwide. Objectives. To describe the antibiotic susceptibility profiles of K. pneumoniae isolates from bacteraemic patients submitted by sentinel laboratories in five regions of South Africa from mid-2010 to mid-2012. Molecular methods were used to detect the most commonly found extended-spectrum beta-lactamase (ESBL) and carbapenemase resistance genes. Methods. Thirteen academic centres serving the public healthcare sector in Gauteng, KwaZulu-Natal, Free State, Limpopo and Western Cape provinces submitted K. pneumoniae isolates from patients with bloodstream infections. Vitek 2 and MicroScan instruments were used for organism identification and susceptibility testing. Multiplex polymerase chain reactions (PCRs) were used to detect blaCTX-M, blaSHV and blaTEM genes in a proportion of the ESBL isolates. All isolates exhibiting reduced susceptibility to carbapenems were PCR tested for blaKPC and blaNDM-1 resistance genes. Results. Overall, 68.3% of the 2 774 isolates were ESBL-positive, showing resistance to cefotaxime, ceftazidime and cefepime. Furthermore, 46.5% of all isolates were resistant to ciprofloxacin and 33.1% to piperacillin-tazobactam. The major ESBL genes were abundantly present in the sample analysed. Most isolates (95.5%) were susceptible to the carbapenems tested, and no isolates were positive for blaKPC or blaNDM-1. There was a trend towards a decrease in susceptibility to most antibiotics. Conclusion. The high proportion of ESBL-producing K. pneumoniae isolates observed, and the prevalence of ESBL genes, are of great concern. Our findings represent a baseline for further surveillance in SA, and can be used for policy and treatment decisions.en_ZA
dc.description.urihttp://www.samj.org.za/index.php/samj/article/view/7617
dc.description.versionPublisher's versionen_ZA
dc.format.extent6 pagesen_ZA
dc.identifier.citationPerovic, O. et al. 2014. National sentinel site surveillance for antimicrobial resistance in Klebsiella pneumoniae isolates in South Africa, 2010-2012. South African Medical Journal, 104(8):563-568, doi:10.7196/SAMJ.7617.en_ZA
dc.identifier.issn2078-5135 (online)
dc.identifier.issn0256-9574 (print)
dc.identifier.otherdoi:10.7196/SAMJ.7617
dc.identifier.urihttp://hdl.handle.net/10019.1/97677
dc.language.isoen_ZAen_ZA
dc.publisherHealth & Medical Publishing Groupen_ZA
dc.rights.holderSouth African Medical Journalen_ZA
dc.subjectDrug resistance in microorganisms -- Statisticsen_ZA
dc.subjectKlebsiella pneumoniaeen_ZA
dc.subjectExtended-spectrum beta-lactamasesen_ZA
dc.subjectDrug resistance in microorganismsen_ZA
dc.titleNational sentinel site surveillance for antimicrobial resistance in Klebsiella pneumoniae isolates in South Africa, 2010-2012en_ZA
dc.typeArticleen_ZA
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