MUC1, a mucin-like protein that is regulated by MSS10, is critical for pseudohyphal differentiation in yeast

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dc.contributor.authorLambrechts, M. G.en_ZA
dc.contributor.authorBauer, Florianen_ZA
dc.contributor.authorMarmur, J.en_ZA
dc.contributor.authorPretorius, I. S.en_ZA
dc.date.accessioned2011-05-15T16:02:21Z
dc.date.available2011-05-15T16:02:21Z
dc.date.issued1996
dc.description.abstractPseudohyphal differentiation in Saccharomyces cerevisiae was first described as a response of diploid cells to nitrogen limitation. Here we report that haploid and diploid starch-degrading S. cerevisiae strains were able to switch from a yeast form to a filamentous pseudohyphal form in response to carbon limitation in the presence of an ample supply of nitrogen. Two genes, MSS10 and MUC1, were cloned and shown to be involved in pseudohyphal differentiation and invasive growth. The deletion of MSS10 resulted in extremely reduced amounts of pseudohyphal differentiation and invasive growth, whereas the deletion of MUC1 abolished pseudohyphal differentiation and invasive growth completely. Mss10 appears to be a transcriptional activator that responds to nutrient limitation and coregulates the expression of MUC1 and the STAI-3 glucoamylase genes, which are involved in starch degradation. MUC1 encodes a 1367-amino acid protein, containing several serine/threonine-rich repeats. Muc1 is a putative integral membrane-bound protein, similar to mammalian mucin-like membrane proteins that have been implicated to play a role in the ability of cancer cells to invade other tissues.
dc.description.versionArticle
dc.identifier.citationProceedings of the National Academy of Sciences of the United States of America
dc.identifier.citation93
dc.identifier.citation16
dc.identifier.issn278424
dc.identifier.other10.1073/pnas.93.16.8419
dc.identifier.urihttp://hdl.handle.net/10019.1/12426
dc.subjectfungal protein
dc.subjectglucan 1,4 alpha glucosidase
dc.subjectmembrane protein
dc.subjectmucin
dc.subjectserine
dc.subjectthreonine
dc.subjectarticle
dc.subjectcontrolled study
dc.subjectfungus growth
dc.subjectfungus hyphae
dc.subjectgene expression
dc.subjectnonhuman
dc.subjectnutrient
dc.subjectpriority journal
dc.subjectsaccharomyces cerevisiae
dc.subjecttransactivation
dc.subjectAmino Acid Sequence
dc.subjectBase Sequence
dc.subjectCA-15-3 Antigen
dc.subjectCarbon
dc.subjectCell Differentiation
dc.subjectCloning, Molecular
dc.subjectDiploidy
dc.subjectDNA Primers
dc.subjectDNA-Binding Proteins
dc.subjectGene Expression Regulation, Fungal
dc.subjectGenes, Fungal
dc.subjectHaploidy
dc.subjectImmediate-Early Proteins
dc.subjectMolecular Sequence Data
dc.subjectMucins
dc.subjectMutagenesis, Insertional
dc.subjectRNA, Messenger
dc.subjectSaccharomyces cerevisiae
dc.subjectSaccharomyces cerevisiae Proteins
dc.subjectSequence Alignment
dc.subjectSequence Homology, Amino Acid
dc.subjectStarch
dc.subjectTranscription, Genetic
dc.subjectFungi
dc.subjectMammalia
dc.subjectSaccharomyces cerevisiae
dc.titleMUC1, a mucin-like protein that is regulated by MSS10, is critical for pseudohyphal differentiation in yeast
dc.typeArticle
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