dc.contributor.author	Nguema-Ona, Eric
dc.contributor.author	Moore, John P.
dc.contributor.author	Fagerstrom, Alexandra D.
dc.contributor.author	Fangel, Jonatan U.
dc.contributor.author	Willats, William G. T.
dc.contributor.author	Hugo, Annatjie
dc.contributor.author	Vivier, Melane A.
dc.date.accessioned	2013-06-26T13:55:02Z
dc.date.available	2013-06-26T13:55:02Z
dc.date.issued	2013-03
dc.date.updated	2013-04-09T07:11:06Z
dc.description	The original publication is available at http://www.biomedcentral.com/1471-2229/13/46	en_ZA
dc.description.abstract	Abstract Background Constitutive expression of Vitis vinifera polygalacturonase-inhibiting protein 1 (Vvpgip1) has been shown to protect tobacco plants against Botrytis cinerea. Evidence points to additional roles for VvPGIP1, beyond the classical endopolygalacturonase (ePG) inhibition mechanism, in providing protection against fungal infection. Gene expression and biochemical datasets previously obtained, in the absence of infection, point to the cell wall, and particularly the xyloglucan component of transgenic VvPGIP1 lines as playing a role in fungal resistance. Results To elucidate the role of wall-associated processes in PGIP-derived resistance pre-infection, a wall profiling analysis, using high-throughput and fractionation techniques, was performed on healthy leaves from wild-type and previously characterized transgenic lines. The cell wall structure profile during development was found to be altered in the transgenic lines assessed versus the wild-type plants. Immunoprofiling revealed subtle changes in pectin and cellulose components and marked changes in the hemicellulose matrix, which showed reduced binding in transgenic leaves of VvPGIP1 expressing plants. Using an enzymatic xyloglucan oligosaccharide fingerprinting technique optimized for tobacco arabinoxyloglucans, we showed that polysaccharides of the XEG-soluble domain were modified in relative abundance for certain oligosaccharide components, although no differences in ion profiles were evident between wild-type and transgenic plants. These changes did not significantly influence plant morphology or normal growth processes compared to wild-type lines. Conclusions VvPGIP1 overexpression therefore results in cell wall remodeling and reorganization of the cellulose-xyloglucan network in tobacco in advance of potential infection.	en_ZA
dc.description.version	Publishers' Version	en_ZA
dc.format.extent	23 p. : ill.
dc.identifier.citation	Nguema-Ona, E. et al. 2013. Overexpression of the grapevine PGIP1 in tobacco results in compositional changes in the leaf arabinoxyloglucan network in the absence of fungal infection.BMC Plant Biology, 13(1):46, doi:10.1186/1471-2229-13-46.	en_ZA
dc.identifier.issn	1471-2229 (online)
dc.identifier.other	doi.org/10.1186/1471-2229-13-46
dc.identifier.uri	http://hdl.handle.net/10019.1/80948
dc.language.iso	en_ZA	en_ZA
dc.language.rfc3066	en_ZA
dc.publisher	BioMed Central	en_ZA
dc.rights.holder	Eric Nguema-Ona et al.; licensee BioMed Central Ltd.	en_ZA
dc.subject	Polygalacturonase-inhibiting protein (PGIP)	en_ZA
dc.subject	Fungal resistance	en_ZA
dc.title	Overexpression of the grapevine PGIP1 in tobacco results in compositional changes in the leaf arabinoxyloglucan network in the absence of fungal infection	en_ZA
dc.type	Article	en_ZA

Overexpression of the grapevine PGIP1 in tobacco results in compositional changes in the leaf arabinoxyloglucan network in the absence of fungal infection

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