Transcriptomic changes associated with husk scald incidence on pomegranate fruit peel during cold storage

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dc.contributor.authorBelay, Zinash A.en_ZA
dc.contributor.authorCaleb, Oluwafemi J.en_ZA
dc.contributor.authorVorster, Alveraen_ZA
dc.contributor.authorVan Heerden, Carelen_ZA
dc.contributor.authorOpara, Umezuruike Linusen_ZA
dc.date.accessioned2022-08-02T08:16:26Z
dc.date.available2022-08-02T08:16:26Z
dc.date.issued2020-05-05
dc.descriptionCITATION: Belay, Z. A. et al. 2020. Transcriptomic changes associated with husk scald incidence on pomegranate fruit peel during cold storage. Food Research International, 135. doi:10.1016/j.foodres.2020.109285en_ZA
dc.descriptionThe original publication is available at https://www.sciencedirect.com/journal/food-research-internationalen_ZA
dc.description.abstractPomegranate fruit is valued for its social, economic, aesthetic and health benefits. The fruit rapidly loses quality after harvest due to continued metabolic responses and physiological disorders under sub-optimal conditions. The incidence of physiological disorder such as husk scald manifests during storage and commercial shipping, which affects the appearance and limits marketability. Despite the importance of pomegranate husk scald, little information is available about the origin and molecular mechanisms. Therefore, the aim of this study was to investigate the scald incidence of pomegranate fruit at molecular level using RNA-Seq (Ion Proton™ Next Generation Sequencing) by analyzing peel transcriptomic changes. The RNA-seq analysis generated 98,441,278 raw reads. 652 Differentially Expressed Genes (DEGs) with a fold change of > |2|, a p value ≤ 0.05 and a false discovery rate (FDR) of <0.05 were identified between healthy and scald fruit peels. An analysis of the gene ontologies of these DEGs revealed the 432 genes were assigned with molecular functions, 272 as cellular components and 205 as part of biological processes. In this analysis, genes (Pgr023188 and Pgr025081) that encode uncharacterized protein and gene (Pgr007593) that encodes glycosyltransferase showed significantly highest fold changes. Genes (Pgr003448, Pgr006024 and Pgr023696) involved in various iron binding and oxidoreductase activities were significantly suppressed. This is the first transcriptome analysis of pomegranate fruit peel related to husk scald development. Results obtained from this study will add valuable information on husk scald related changes on pomegranate fruit at genomic level and provide insight on other related physiological disorders.en_ZA
dc.description.urihttps://www.sciencedirect.com/science/article/pii/S0963996920303100?via%3Dihub
dc.description.versionPublishers versionen_ZA
dc.format.extent12 pages : illustrationsen_ZA
dc.identifier.citationBelay, Z. A. et al. 2020. Transcriptomic changes associated with husk scald incidence on pomegranate fruit peel during cold storage. Food Research International, 135. doi:10.1016/j.foodres.2020.109285en_ZA
dc.identifier.issn0963-9969 (print)
dc.identifier.otherdoi:10.1016/j.foodres.2020.109285
dc.identifier.urihttp://hdl.handle.net/10019.1/125568
dc.language.isoen_ZAen_ZA
dc.publisherElsevieren_ZA
dc.rights.holderElsevieren_ZA
dc.subjectPhysiological disorders in pomegranateen_ZA
dc.subjectGene expressionen_ZA
dc.subjectPomegranate storageen_ZA
dc.subjectPomegranate -- Postharvest biotechnologyen_ZA
dc.subjectPomegranate --Postharvest handlingen_ZA
dc.titleTranscriptomic changes associated with husk scald incidence on pomegranate fruit peel during cold storageen_ZA
dc.typeArticleen_ZA
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