Metabolomic alterations do not induce metabolic burden in the industrial yeast M2n[pBKD2-Pccbgl1]-C1 engineered by multiple δ-integration of a fungal β-glucosidase gene

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dc.contributor.authorFavaro, Lorenzoen_ZA
dc.contributor.authorCagnin, Lorenzoen_ZA
dc.contributor.authorCorte, Lauraen_ZA
dc.contributor.authorRoscini, Lucaen_ZA
dc.contributor.authorDe Pascale, Fabioen_ZA
dc.contributor.authorTreu, Lauraen_ZA
dc.contributor.authorCampanaro, Stefanoen_ZA
dc.contributor.authorBasaglia, Marinaen_ZA
dc.contributor.authorVan Zyl, Willem H.en_ZA
dc.contributor.authorCasella, Sergioen_ZA
dc.contributor.authorCardinali, Gianluigien_ZA
dc.date.accessioned2021-09-15T07:04:43Z
dc.date.available2021-09-15T07:04:43Z
dc.date.issued2019-11-28
dc.descriptionCITATION: Favaro L., et al. 2019. Metabolomic alterations do not induce metabolic burden in the industrial yeast M2n[pBKD2-Pccbgl1]-C1 engineered by multiple d-integration of a fungal b-glucosidase gene. Frontiers in Bioengineering and Biotechnology. 7:376. doi:10.3389/fbioe.2019.00376
dc.descriptionThe original publication is available at https://www.frontiersin.org/articles/10.3389/fbioe.2019.00376/full
dc.description.abstractIn the lignocellulosic yeast development, metabolic burden relates to redirection of resources from regular cellular activities toward the needs created by recombinant protein production. As a result, growth parameters may be greatly affected. Noteworthy, Saccharomyces cerevisiae M2n[pBKD2-Pccbgl1]-C1, previously developed by multiple d-integration of the b-glucosidase BGL3, did not show any detectable metabolic burden. This work aims to test the hypothesis that the metabolic burden and the metabolomic perturbation induced by the d-integration of a yeast strain, could differ significantly. The engineered strain was evaluated in terms of metabolic performances and metabolomic alterations in different conditions typical of the bioethanol industry. Results indicate that the multiple d-integration did not affect the ability of the engineered strain to grow on different carbon sources and to tolerate increasing concentrations of ethanol and inhibitory compounds. Conversely, metabolomic profiles were significantly altered both under growing and stressing conditions, indicating a large extent of metabolic reshuffling involved in the maintenance of the metabolic homeostasis. Considering that four copies of BGL3 gene have been integrated without affecting any parental genes or promoter sequences, deeper studies are needed to unveil the mechanisms implied in these metabolomic changes, thus supporting the optimization of protein production in engineered strains.en_ZA
dc.description.sponsorshipPadova University
dc.description.urihttps://www.frontiersin.org/articles/10.3389/fbioe.2019.00376/full
dc.description.versionPublisher’s version
dc.format.extent13 pages
dc.identifier.citationFavaro L., et al. 2019. Metabolomic alterations do not induce metabolic burden in the industrial yeast M2n[pBKD2-Pccbgl1]-C1 engineered by multiple d-integration of a fungal b-glucosidase gene. Frontiers in Bioengineering and Biotechnology. 7:376. doi:10.3389/fbioe.2019.00376
dc.identifier.issn2296-4185 (online)
dc.identifier.otherdoi:10.3389/fbioe.2019.00376
dc.identifier.urihttp://hdl.handle.net/10019.1/123027
dc.language.isoen_ZAen_ZA
dc.publisherFrontiers Media
dc.rights.holderAuthors retain copyright
dc.subjectLignocellulosic ethanolen_ZA
dc.subjectMetabolic burdenen_ZA
dc.subjectMetabolomic fingerprinten_ZA
dc.subjectFourier transform infrared spectroscopyen_ZA
dc.subjectStress responseen_ZA
dc.subjectSaccharomyces cerevisiae -- Effect of stress onen_ZA
dc.subjectBeta-glucuronidase genes -- Inhibitorsen_ZA
dc.titleMetabolomic alterations do not induce metabolic burden in the industrial yeast M2n[pBKD2-Pccbgl1]-C1 engineered by multiple δ-integration of a fungal β-glucosidase geneen_ZA
dc.typeArticleen_ZA
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