Many saccharomyces cerevisiae cell wall protein encoding genes are coregulated by Mss11, but cellular adhesion phenotypes appear only flo protein dependent

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dc.contributor.authorBester, Michael C.en_ZA
dc.contributor.authorJacobson, Danen_ZA
dc.contributor.authorBauer, Florianen_ZA
dc.date.accessioned2012-04-25T13:19:55Z
dc.date.available2012-04-25T13:19:55Z
dc.date.issued2012-01
dc.descriptionThe original publication is available at http://www.g3journal.org/en_ZA
dc.descriptionPublication of this article was funded by the Stellenbosch University Open Access Fund.en_ZA
dc.description.abstractThe outer cell wall of the yeast Saccharomyces cerevisiae serves as the interface with the surrounding environment and directly affects cell2cell and cell2surface interactions. Many of these interactions are facilitated by specific adhesins that belong to the Flo protein family. Flo mannoproteins have been implicated in phenotypes such as flocculation, substrate adhesion, biofilm formation, and pseudohyphal growth. Genetic data strongly suggest that individual Flo proteins are responsible for many specific cellular adhesion phenotypes. However, it remains unclear whether such phenotypes are determined solely by the nature of the expressed FLO genes or rather as the result of a combination of FLO gene expression and other cell wall properties and cell wall proteins. Mss11 has been shown to be a central element of FLO1 and FLO11 gene regulation and acts together with the cAMP-PKA-dependent transcription factor Flo8. Here we use genome-wide transcription analysis to identify genes that are directly or indirectly regulated by Mss11. Interestingly, many of these genes encode cell wall mannoproteins, in particular, members of the TIR and DAN families. To examine whether these genes play a role in the adhesion properties associated with Mss11 expression, we assessed deletion mutants of these genes in wild-type and flo11D genetic backgrounds. This analysis shows that only FLO genes, in particular FLO1/10/11, appear to significantly impact on such phenotypes. Thus adhesion-related phenotypes are primarily dependent on the balance of FLO gene expression.en_ZA
dc.description.sponsorshipStellenbosch University Open Access Funden_ZA
dc.description.versionPublishers' Versionen_ZA
dc.format.extentp. 131-141 : ill. (some col.)en_ZA
dc.identifier.citationBester, M., Jacobson, D. & Bauer, F. F. 2012. Many saccharomyces cerevisiae cell wall protein encoding genes are coregulated by Mss11, but cellular adhesion phenotypes appear only flo protein dependent. G3, Genomes, Genetics, 2(1), 131-141, doi:10.1534/g3.111.001644.en_ZA
dc.identifier.issn2160-1836 (online)
dc.identifier.otherdoi:10.1534/g3.111.001644
dc.identifier.urihttp://hdl.handle.net/10019.1/20868
dc.language.isoen_ZAen_ZA
dc.publisherGenetics Society of Americaen_ZA
dc.rights.holderAuthor retain the copyrighten_ZA
dc.subjectFlocculationen_ZA
dc.subjectFLO genesen_ZA
dc.subjectCellular adhesionen_ZA
dc.subjectSaccharomyces cerevisiaeen_ZA
dc.titleMany saccharomyces cerevisiae cell wall protein encoding genes are coregulated by Mss11, but cellular adhesion phenotypes appear only flo protein dependenten_ZA
dc.typeArticleen_ZA
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