Development and evaluation of a diagnostic cytokine-release assay for mycobacterium suricattae infection in meerkats (Suricata suricatta)

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dc.contributor.authorClarke, Charleneen_ZA
dc.contributor.authorPatterson, Stuart Jamesen_ZA
dc.contributor.authorDrewe, Julian Ashleyen_ZA
dc.contributor.authorVan Helden, Paul Daviden_ZA
dc.contributor.authorMiller, Michele Annen_ZA
dc.contributor.authorParsons, Sven David Charlesen_ZA
dc.date.accessioned2017-01-17T12:28:25Z
dc.date.available2017-01-17T12:28:25Z
dc.date.issued2017-01-04
dc.date.updated2017-01-04T07:13:22Z
dc.descriptionCITATION: Clarke, C., et al. 2017. Development and evaluation of a diagnostic cytokine-release assay for mycobacterium suricattae infection in meerkats (Suricata suricatta). BMC Veterinary Research, 13:2, doi:10.1186/s12917-016-0927-x.
dc.descriptionThe original publication is available at http://bmcvetres.biomedcentral.com
dc.description.abstractBackground: Sensitive diagnostic tools are necessary for the detection of Mycobacterium suricattae infection in meerkats (Suricata suricatta) in order to more clearly understand the epidemiology of tuberculosis and the ecological consequences of the disease in this species. We therefore aimed to develop a cytokine release assay to measure antigen-specific cell-mediated immune responses of meerkats. Results: Enzyme-linked immunosorbent assays (ELISAs) were evaluated for the detection of interferon-gamma (IFN-γ) and IFN-γ inducible protein 10 (IP-10) in meerkat plasma. An IP-10 ELISA was selected to measure the release of this cytokine in whole blood in response to Bovigam® PC-HP Stimulating Antigen, a commercial peptide pool of M. bovis antigens. Using this protocol, captive meerkats with no known M. suricattae exposure (n = 10) were tested and results were used to define a diagnostic cut off value (mean plus 2 standard deviations). This IP-10 release assay (IPRA) was then evaluated in free-living meerkats with known M. suricattae exposure, categorized as having either a low, moderate or high risk of infection with this pathogen. In each category, respectively, 24.7%, 27.3% and 82.4% of animals tested IPRA-positive. The odds of an animal testing positive was 14.0 times greater for animals with a high risk of M. suricattae infection compared to animals with a low risk. Conclusion: These results support the use of this assay as a measure of M. suricattae exposure in meerkat populations. Ongoing longitudinal studies aim to evaluate the value of the IPRA as a diagnostic test of M. suricattae infection in individual animals.en_ZA
dc.description.urihttp://bmcvetres.biomedcentral.com/articles/10.1186/s12917-016-0927-x
dc.description.versionPublisher's version
dc.format.extent6 pages : illustrationsen_ZA
dc.identifier.citationClarke, C., et al. 2017. Development and evaluation of a diagnostic cytokine-release assay for mycobacterium suricattae infection in meerkats (Suricata suricatta). BMC Veterinary Research, 13:2, doi:10.1186/s12917-016-0927-x
dc.identifier.issn1746-6148 (online)
dc.identifier.otherdoi:10.1186/s12917-016-0927-x
dc.identifier.urihttp://hdl.handle.net/10019.1/100472
dc.language.isoen_ZAen_ZA
dc.publisherBioMed Centralen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectMycobacterium suricattae infectionen_ZA
dc.subjectCytokinesen_ZA
dc.subjectCytologic diagnosisen_ZA
dc.subjectMeerkaten_ZA
dc.subjectMycobacterium infectionsen_ZA
dc.subjectSuricata suricattaen_ZA
dc.titleDevelopment and evaluation of a diagnostic cytokine-release assay for mycobacterium suricattae infection in meerkats (Suricata suricatta)en_ZA
dc.typeArticle
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