Continual proteomic divergence of HepG2 cells as a consequence of long-term spheroid culture

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dc.contributor.authorEllero, Andrea Antonioen_ZA
dc.contributor.authorVan den Bout, Imanen_ZA
dc.contributor.authorVlok, Mareen_ZA
dc.contributor.authorCromarty, Allan Duncanen_ZA
dc.contributor.authorHurrell, Traceyen_ZA
dc.date.accessioned2023-04-04T06:19:16Z
dc.date.available2023-04-04T06:19:16Z
dc.date.issued2021
dc.descriptionCITATION: Ellero, A. A. et al. 2021. Continual proteomic divergence of HepG2 cells as a consequence of long-term spheroid culture. Scientific Reports, 11:10917, doi:10.1038/s41598-021-89907-9.
dc.descriptionThe original publication is available at https://www.nature.com
dc.description.abstractThree-dimensional models are considered a powerful tool for improving the concordance between in vitro and in vivo phenotypes. However, the duration of spheroid culture may influence the degree of correlation between these counterparts. When using immortalised cell lines as model systems, the assumption for consistency and reproducibility is often made without adequate characterization or validation. It is therefore essential to define the biology of each spheroid model by investigating proteomic dynamics, which may be altered relative to culture duration. As an example, we assessed the influence of culture duration on the relative proteome abundance of HepG2 cells cultured as spheroids, which are routinely used to model aspects of the liver. Quantitative proteomic profiling of whole cell lysates labelled with tandem-mass tags was conducted using liquid chromatography-tandem mass spectrometry (LC–MS/MS). In excess of 4800 proteins were confidently identified, which were shared across three consecutive time points over 28 days. The HepG2 spheroid proteome was divergent from the monolayer proteome after 14 days in culture and continued to change over the successive culture time points. Proteins representing the recognised core hepatic proteome, cell junction, extracellular matrix, and cell adhesion proteins were found to be continually modulated.en_ZA
dc.description.urihttps://www.nature.com/articles/s41598-021-89907-9
dc.description.versionPublisher's version
dc.format.extent14 pages : illustrations (some color)en_ZA
dc.identifier.citationEllero, A. A. et al. 2021. Continual proteomic divergence of HepG2 cells as a consequence of long-term spheroid culture. Scientific Reports, 11:10917, doi:10.1038/s41598-021-89907-9.
dc.identifier.issn2045-2322 (online)
dc.identifier.otherdoi:10.1038/s41598-021-89907-9
dc.identifier.urihttp://hdl.handle.net/10019.1/126767
dc.language.isoen_ZAen_ZA
dc.publisherNature
dc.rights.holderAuthors retain copyright
dc.subjectHepG2 cellsen_ZA
dc.subjectProteomics -- Dynamicsen_ZA
dc.subjectPhenotypeen_ZA
dc.subjectLiver cells -- Effect of drugs onen_ZA
dc.subjectSpheroidsen_ZA
dc.titleContinual proteomic divergence of HepG2 cells as a consequence of long-term spheroid cultureen_ZA
dc.typeArticleen_ZA
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