Mycosin-1, a subtilisin-like serine protease of Mycobacterium tuberculosis, is cell wall-associated and expressed during infection of macrophages

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dc.contributor.authorDave, Joel A.
dc.contributor.authorGey van Pittius, Nico C.
dc.contributor.authorBeyers, Albert D.
dc.contributor.authorEhlers, Mario R. W.
dc.contributor.authorBrown, Gordon D.
dc.date.accessioned2010-12-14T09:04:41Z
dc.date.available2010-12-14T09:04:41Z
dc.date.issued2002-10
dc.date.updated2010-11-09T19:07:12Z
dc.descriptionIncludes bibliography
dc.description.abstractBackground: Exported proteases are commonly associated with virulence in bacterial pathogens, yet there is a paucity of information regarding their role in Mycobacterium tuberculosis. There are five genes (mycP1-5) present within the genome of Mycobacterium tuberculosis H37Rv that encode a family of secreted, subtilisin-like serine proteases (the mycosins). The gene mycP1 (encoding mycosin-1) was found to be situated 3700 bp (four ORF's) from the RD1 deletion region in the genome of the attenuated vaccine strain M. bovis BCG (bacille de Calmette et Guérin) and was selected for further analyses due to the absence of expression in this organism. Results: Full-length, 50 kDa mycosin-1 was observed in M. tuberculosis cellular lysates, whereas lower-molecular-weight species were detected in culture filtrates. A similar lower-molecular-weight species was also observed during growth in macrophages. Mycosin-1 was localized to the membrane and cell wall fractions in M. tuberculosis by Western blotting, and to the cell envelope by electron microscopy. Furthermore, M. tuberculosis culture filtrates were shown to contain a proteolytic activity inhibited by mixed serine/cysteine protease inhibitors and activated by Ca2+, features typical of the subtilisins. Conclusions: Mycosin-1 is an extracellular protein that is membrane- and cell wall-associated, and is shed into the culture supernatant. The protein is expressed after infection of macrophages and is subjected to proteolytic processing. Although proteolytically active mycosin-1 could not be generated recombinantly, serine protease activity containing features typical of the subtilisins was detected in M. tuberculosis culture filtrates.en_ZA
dc.description.versionPeer Reviewed
dc.format.extent8 p. : ill.
dc.identifier.citationDave, JA, Gey van Pittius, NC, Beyers, AD, Ehlers, MRW & Brown, GD 2002, 'Mycosin-1, a subtilisin-like serine protease of Mycobacterium tuberculosis, is cell wall-associated and expressed during infection of macrophages', BMC Microbiology, 2(1):30.en_ZA
dc.identifier.issn1471-2180
dc.identifier.otherhttp://dx.doi.org/10.1186/1471-2180-2-30
dc.identifier.urihttp://hdl.handle.net/10019.1/5089
dc.language.isoen_ZAen_ZA
dc.language.rfc3066en
dc.publisherBioMed Centralen_ZA
dc.rights.holderDave et al.; licensee BioMed Central Ltd.en_ZA
dc.subjectExported proteasesen_ZA
dc.subjectMycobacterium tuberculosisen_ZA
dc.subjectMacrophagus -- Activation
dc.titleMycosin-1, a subtilisin-like serine protease of Mycobacterium tuberculosis, is cell wall-associated and expressed during infection of macrophagesen_ZA
dc.typeArticleen_ZA
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