Transformation of potato (cv. Late Harvest) with the potato leafroll virus coat protein gene, and molecular analysis of transgenic lines

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dc.contributor.authorMurray, S. L.en_ZA
dc.contributor.authorBurger, J. T.en_ZA
dc.contributor.authorOelofse, D.en_ZA
dc.contributor.authorCress, W. A.en_ZA
dc.contributor.authorVan Staden, J.en_ZA
dc.contributor.authorBerger, D. K.en_ZA
dc.date.accessioned2011-05-15T15:55:21Z
dc.date.available2011-05-15T15:55:21Z
dc.date.issued1998
dc.descriptionCITATION: Murray, S. L., et al. 1998. Transformation of potato (cv. Late Harvest) with the potato leafroll virus coat protein gene, and molecular analysis of transgenic lines. South African Journal of Science, 94(6):263-268.
dc.descriptionThe original publication is available at https://journals.co.za
dc.description.abstractPotato leafroll virus (PLRV) is one of the most destructive potato viruses in South Africa. In order to establish resistance against PLRV in the potato cultivar Late Harvest, the coat protein (CP) gene of a South African isolate of the virus was isolated, cloned into the plant transformation vector pBI121 and inserted into potatoes using Agrobacterium tumefaciens-mediated transformation. Six plantlets, which appeared to be phenotypically normal, were regenerated from leaf disks under kanamycin selection. These lines were analysed for stable transgene insertion and expression. The presence of the PLRV CP, uidA (GUS) and nptII (kanamycin resistance) genes were shown using PCR! Southern blot analysis verified that the PLRV CP gene had been inserted into the genome of the transgenic potato lines. Coat protein could not be detected, but RNA dot blots demonstrated PLRV CP gene expression at the mRNA level. Expression of the uidA gene was investigated using a fluorometric assay, and it was observed that lines containing the PLRV CP gene in the antisense orientation exhibited GUS activity.
dc.description.urihttps://journals.co.za/content/sajsci/94/6/AJA00382353_7730
dc.description.versionPublisher's version
dc.format.extent6 pages
dc.identifier.citationMurray, S. L., et al. 1998. Transformation of potato (cv. Late Harvest) with the potato leafroll virus coat protein gene, and molecular analysis of transgenic lines. South African Journal of Science, 94(6):263-268
dc.identifier.issn1996-7489 (online)
dc.identifier.issn0038-2353 (print)
dc.identifier.urihttp://hdl.handle.net/10019.1/9726
dc.language.isoen_ZAen_ZA
dc.publisherAcademy of Science for South Africa
dc.rights.holderAcademy of Science for South Africa
dc.subjectTransgenic plants
dc.subjectPotato leafroll virus
dc.titleTransformation of potato (cv. Late Harvest) with the potato leafroll virus coat protein gene, and molecular analysis of transgenic linesen_ZA
dc.typeArticle
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