Carnitine biosynthesis in Neurospora crassa: Identification of a cDNA coding for ε-N-trimethyllysine hydroxylase and its functional expression in Saccharomyces cerevisiae

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dc.contributor.authorSwiegers, J. H.en_ZA
dc.contributor.authorVaz, F.M.en_ZA
dc.contributor.authorPretorius, I. S.en_ZA
dc.contributor.authorWanders, R. J. Aen_ZA
dc.contributor.authorBauer, Florianen_ZA
dc.date.accessioned2011-05-15T16:04:57Z
dc.date.available2011-05-15T16:04:57Z
dc.date.issued2002
dc.description.abstractThe biosynthesis of L-carnitine in eukaryotic organisms was first elucidated in the ascomycete Neurospora crassa. The first step of the pathway is catalysed by ε-N-trimethyllysine hydroxylase (TMLH), which converts ε-N-trimethyllysine into β-hydroxy-N-ε-trimethyllysine in a reaction dependent on α-ketoglutarate, Fe2+ and oxygen. Here we report on the cloning of the N. crassa TMLH cDNA and its functional expression in Saccharomyces cerevisiae. The TMLH cDNA contains an open reading frame of 1413 base pairs encoding a predicted polypeptide of 471 amino acids. The Michaelis-Menten constants of the heterologously expressed enzyme were determined for ε-N-trimethyllysine, α-ketoglutarate, Fe2+ and correspond to 0.33 mM, 133 μM and 46 μM, respectively. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
dc.description.versionArticle
dc.identifier.citationFEMS Microbiology Letters
dc.identifier.citation210
dc.identifier.citation1
dc.identifier.issn3781097
dc.identifier.other10.1016/S0378-1097(02)00520-7
dc.identifier.urihttp://hdl.handle.net/10019.1/12900
dc.subject2 oxoglutaric acid
dc.subject6 n trimethyllysine hydroxylase
dc.subjectcarnitine
dc.subjectcomplementary DNA
dc.subjectiron
dc.subjectoxygenase
dc.subjectunclassified drug
dc.subjectarticle
dc.subjectbase pairing
dc.subjectbiosynthesis
dc.subjectcontrolled study
dc.subjectmolecular cloning
dc.subjectNeurospora crassa
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectopen reading frame
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectSaccharomyces cerevisiae
dc.subjectAmino Acid Sequence
dc.subjectCarnitine
dc.subjectCloning, Molecular
dc.subjectConsensus Sequence
dc.subjectDNA, Complementary
dc.subjectKinetics
dc.subjectMixed Function Oxygenases
dc.subjectMolecular Sequence Data
dc.subjectNeurospora crassa
dc.subjectSaccharomyces cerevisiae
dc.subjectSequence Alignment
dc.subjectSequence Homology, Amino Acid
dc.subjectEukaryota
dc.subjectNeurospora
dc.subjectNeurospora crassa
dc.subjectSaccharomyces
dc.subjectSaccharomyces cerevisiae
dc.titleCarnitine biosynthesis in Neurospora crassa: Identification of a cDNA coding for ε-N-trimethyllysine hydroxylase and its functional expression in Saccharomyces cerevisiae
dc.typeArticle
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