Measuring thiols in single cultivar South African red wines using 4,4-dithiodipyridine (DTDP) derivatization and ultraperformance convergence chromatography-tandem mass spectrometry
Date
2018
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI
Abstract
Wine varietal thiols are important contributors to wine aroma. The chemical nature of
thiols makes them difficult to measure due to low concentrations, high sensitivity to oxidation, and
low ionization. Methods for the measurement of thiols usually consist of multiple steps of sample
preparation followed by instrumental measurement. Studies have collected large datasets of thiols
in white wine but not in red wine, due to the lack of availability of suitable methods. In this study,
for the first time, convergence chromatography was used to measure thiols in red wine at ultratrace
levels with improved sensitivity compared to previous methods. Performance parameters (selectivity,
linearity, limits of detection, precision, accuracy) were tested to demonstrate the suitability of the
method for the proposed application. Red wine thiols were measured in South African Pinotage,
Shiraz, and Cabernet Sauvignon wines (n = 16 each). Cultivar differentiation using the thiol profile
was demonstrated.
Description
CITATION: Mafata, M. et al. 2018. Measuring thiols in single cultivar South African red wines using 4,4-dithiodipyridine (DTDP) derivatization and ultraperformance convergence chromatography-tandem mass spectrometry. Foods, 7(9):138, doi:10.3390/foods7090138.
The original publication is available at https://www.mdpi.com
The original publication is available at https://www.mdpi.com
Keywords
Thiols, Wine and wine making -- South Africa, Mass spectrometry, Chromatographic analysis
Citation
Mafata, M. et al. 2018. Measuring thiols in single cultivar South African red wines using 4,4-dithiodipyridine (DTDP) derivatization and ultraperformance convergence chromatography-tandem mass spectrometry. Foods, 7(9):138, doi:10.3390/foods7090138.