The effect of increased branched-chain amino acid transaminase activity in yeast on the production of higher alcohols and on the flavour profiles of wine and distillates
Date
2006
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Abstract
In Saccharomyces cerevisiae, branched-chain amino acid transaminases (BCAAT ases) are encoded by the BAT1 and BAT2 genes. BCAATases catalyse the transfer of amino groups between those amino acids and α-keto-acids, α-Keto-acids are precursors for the biosynthesis of higher alcohols, which significantly influence the aroma and flavour of yeast-derived fermentation products. The objective of this study was to investigate the influence of BAT-gene expression on general yeast physiology, on aroma and flavour compound formation and on the sensory characteristics of wines and distillates. For this purpose, the genes were overexpressed and deleted in a laboratory strain, BY4742, and overexpressed in an industrial wine yeast strain, VIN13. The data show that, with the exception of a slow growth phenotype observed for the BAT1 deletion strain, the fermentation behaviour of the strains was unaffected by the modifications. The chemical and sensory analysis of fermentation products revealed a strong correction between BAT gene expression and the formation of many aroma compounds. The data suggest that the adjustment of BAT gene expression could play an important role in assisting winemakers in their endeavour to produce wines with specific flavour profiles. © 2006 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved.
Description
Keywords
branched chain amino acid aminotransferase, flavoring agent, fungal enzyme, adjustment, alcohol production, aroma, article, BAT1 gene, BAT2 gene, behavior, chemical analysis, enzyme activity, fungal strain, gene, gene deletion, gene expression, nonhuman, phenotype, physiology, sensory analysis, wine, yeast, Alcohols, Fermentation, Flavoring Agents, Mitochondrial Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transaminases, Wine, Saccharomyces cerevisiae
Citation
FEMS Yeast Research
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