2'-5'-Oligoadenylate synthetase-like protein inhibits intracellular M. tuberculosis replication and promotes proinflammatory cytokine secretion

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dc.contributor.authorLeisching, G.en_ZA
dc.contributor.authorAli, A.en_ZA
dc.contributor.authorCole, V.en_ZA
dc.contributor.authorBaker, B.en_ZA
dc.date.accessioned2023-01-20T07:13:47Zen_ZA
dc.date.available2023-01-20T07:13:47Zen_ZA
dc.date.issued2019-12en_ZA
dc.descriptionCITATION: Leisching, G. et al. 2020. 2'-5'-Oligoadenylate synthetase-like protein inhibits intracellular M. tuberculosis replication and promotes proinflammatory cytokine secretion. Molecular Immunology, 118:73–78, doi:0.1016/j.molimm.2019.12.004.en_ZA
dc.descriptionThe original publication is available at https://www.sciencedirect.comen_ZA
dc.description.abstractHost cytoplasmic surveillance pathways are known to elicit type I interferon (IFN) responses which are crucial to antimicrobial defense mechanisms. Oligoadenylate synthetase-like (OASL) protein has been extensively characterized as a part of the anti-viral mechanism, however a number of transcriptomic studies reveal its upregulation in response to infection with a wide variety of intracellular bacterial pathogens. To date, there is no evidence documenting the role (if any) of OASL during mycobacterium tuberculosis infection. Using two pathogenic strains differing in virulence only, as well as the non-pathogenic M. bovis BCG strain, we observed that pathogenicity and virulence strongly induced OASL expression after 24 h of infection. Further, we observed that OASL knock down led to a significant increase in M. tb CFU counts 96 h post-infection in comparison to the respective controls. Luminex revealed that OASL silencing significantly decreased IL-1β, TNF-α and MCP-1 secretion in THP-1 cells and had no effect on IL-10 secretion. We therefore postulate that OASL regulates pro-inflammatory mediators such as cytokines and chemokines which suppress intracellular mycobacterial growth and survival.en_ZA
dc.description.sponsorshipSouth African Medical Research Councilen_ZA
dc.description.sponsorshipNational Research Foundation of South Africaen_ZA
dc.description.versionPublisher's versionen_ZA
dc.format.extent6 pagesen_ZA
dc.identifier.citationLeisching, G. et al. 2020. 2'-5'-Oligoadenylate synthetase-like protein inhibits intracellular M. tuberculosis replication and promotes proinflammatory cytokine secretion. Molecular Immunology, 118:73–78, doi:0.1016/j.molimm.2019.12.004.en_ZA
dc.identifier.issn1872-9142 (online)en_ZA
dc.identifier.issn0161-5890 (print)en_ZA
dc.identifier.otherdoi:0.1016/j.molimm.2019.12.004en_ZA
dc.identifier.urihttp://hdl.handle.net/10019.1/126266en_ZA
dc.language.isoen_ZAen_ZA
dc.publisherElsevieren_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectMycobacterium tuberculosisen_ZA
dc.subjectOligoadenylate synthetaseen_ZA
dc.subjectCytokines -- Secretionen_ZA
dc.subjectIntracellular pathogensen_ZA
dc.subjectAnti-infective agentsen_ZA
dc.subjectNatural immunityen_ZA
dc.title2'-5'-Oligoadenylate synthetase-like protein inhibits intracellular M. tuberculosis replication and promotes proinflammatory cytokine secretionen_ZA
dc.typeArticleen_ZA
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