Biofilm formation in invasive Staphylococcus aureus isolates is associated with the clonal lineage

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dc.contributor.advisorNaicker, Preneshni Rochelleen_ZA
dc.contributor.advisorKarayem, Karayemen_ZA
dc.contributor.authorHoek, Kim G. P.en_ZA
dc.contributor.authorHarvey, Justinen_ZA
dc.contributor.authorWasserman, Elizabethen_ZA
dc.date.accessioned2015-01-13T11:46:11Z
dc.date.available2015-10-01T03:00:08Z
dc.date.issued2016-01en_ZA
dc.descriptionPlease cite as follows: Naicker, P. R. et al. 2016. Biofilm formation in invasive Staphylococcus aureus isolates is associated with the clonal lineage. Microbial Pathogenesis, 90:41-49, doi:10.1016/j.micpath.2015.10.023.en_ZA
dc.descriptionThe original publication is available at http://www.journals.elsevier.com/microbial-pathogenesis/en_ZA
dc.description.abstractENGLISH ABSTRACT: Objectives: The ability to form biofilms contributes significantly to the virulence of Staphylococcus aureus. Virulence factors may be associated with certain S. aureus lineages. However, the contribution of the genetic background of S. aureus to biofilm formation is poorly understood. This study investigated the association between the genetic background and the biofilm forming ability of clinical invasive S. aureus isolates. Secondary objectives included investigating any correlation with biofilm formation and methicillin resistance or the source of bacteraemia. Methods: The study was conducted at a 1300-bed tertiary hospital in Cape Town, South Africa. S. aureus isolates obtained from blood cultures between January 2010 and January 2012 were included. Genotypic characterization was performed by PFGE, spa typing, SCCmec typing and MLST. Thirty genotypically unique strains were assessed for phenotypic biofilm formation with the microtitre plate assay. All isolates were tested in triplicate and an average optical density, measured at a wavelength of 490nm, was determined. The biofilm forming ability of isolates with A490 > 0.17 was considered ‘weak positive’ and A490 > 0.34 ‘strong positive’. Isolates with A490 ≤ 0.17 were considered non-adherent. ANOVA with Bonferroni-adjusted post-hoc tests and t-tests were used for statistical analysis of the association between biofilm forming ability and strain characteristics. Results: Fifty seven percent of isolates were capable of forming biofilms. Weak biofilm formation occurred in 40% (n=12) and strong biofilm formation in 17% (n=5) of isolates. Thirteen (43%) of the isolates were classified as non-adherent. All 5 isolates capable of strong biofilm formation belong to one spa clonal complex (spa-CC 064). Strains from spa-CC 064 were capable of higher biofilm formation than other spa clonal complexes (p=0.00002). These 5 strains belonged to MLST CC5 and CC8. Biofilm formation did not correlate with methicillin resistance and was not related to the source of bacteraemia. Conclusion: Biofilm formation correlates with the spa clonal lineage in our population of invasive S. aureus strains. High biofilm formation was associated with spa-CC 064. MLST CC5 and CC8 strains are capable of strong biofilm formation.en_ZA
dc.description.versionPre-printen_ZA
dc.description.versionPre print
dc.format.extent24 pagesen_ZA
dc.identifier.citationNaicker, P. R. et al. 2016. Biofilm formation in invasive Staphylococcus aureus isolates is associated with the clonal lineage. Microbial Pathogenesis, 90:41-49, doi:10.1016/j.micpath.2015.10.023.en_ZA
dc.identifier.issn1096-1208 (online)en_ZA
dc.identifier.issn0882-4010 (print)en_ZA
dc.identifier.otherdoi:10.1016/j.micpath.2015.10.023en_ZA
dc.identifier.urihttp://hdl.handle.net/10019.1/95723
dc.language.isoen_ZAen_ZA
dc.publisherStellenbosch : Stellenbosch Universityen_ZA
dc.publisherElsevier
dc.rights.holderStellenbosch Universityen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectBiofilmsen_ZA
dc.subjectStaphylococcus aureusen_ZA
dc.subjectStaphylococcus aureus -- Genetic aspectsen_ZA
dc.titleBiofilm formation in invasive Staphylococcus aureus isolates is associated with the clonal lineageen_ZA
dc.typeArticleen_ZA
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