Recent developments in the application of flow cytometry to advance our understanding of Mycobacterium tuberculosis physiology and pathogenesis

Simple item page
dc.contributor.authorParbhoo, Trishaen_ZA
dc.contributor.authorSampson, Samantha L.en_ZA
dc.contributor.authorMouton, Jacoba M.en_ZA
dc.date.accessioned2022-06-21T10:05:39Zen_ZA
dc.date.available2022-06-21T10:05:39Zen_ZA
dc.date.issued2020-05-21en_ZA
dc.descriptionCITATION: Parbhoo, T., Sampson, S.L. & Mouton, J.M. 2020. Recent developments in the application of flow cytometry to advance our understanding of Mycobacterium tuberculosis physiology and pathogenesis. Cytometry Part A, 97(7):683-693. doi:10.1002/cyto.a.24030en_ZA
dc.descriptionThe original publication is available at https://onlinelibrary.wiley.com/journal/15524930en_ZA
dc.description.abstractThe ability of the bacterial pathogen Mycobacterium tuberculosis to adapt and survive within human cells to disseminate to other individuals and cause active disease is poorly understood. Research supports that as M. tuberculosis adapts to stressors encountered in the host, it exhibits variable physiological and metabolic states that are time and niche-dependent. Challenges associated with effective treatment and eradication of tuberculosis (TB) are in part attributed to our lack of understanding of these different mycobacterial phenotypes. This is mainly due to a lack of suitable tools to effectively identify/detect heterogeneous bacterial populations, which may include small, difficult-to-culture subpopulations. Importantly, flow cytometry allows rapid and affordable multiparametric measurements of physical and chemical characteristics of single cells, without the need to preculture cells. Here, we summarize current knowledge of flow cytometry applications that have advanced our understanding of the physiology of M. tuberculosis during TB disease. Specifically, we review how host-associated stressors influence bacterial characteristics such as metabolic activity, membrane potential, redox status and the mycobacterial cell wall. Further, we highlight that flow cytometry offers unprecedented opportunities for insight into bacterial population heterogeneity, which is increasingly appreciated as an important determinant of disease outcome. © 2020 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry.en_ZA
dc.description.urihttps://onlinelibrary.wiley.com/journal/15524930en_ZA
dc.description.versionPublishers versionen_ZA
dc.format.extent11 pagesen_ZA
dc.identifier.citationParbhoo, T., Sampson, S.L. & Mouton, J.M. 2020. Recent developments in the application of flow cytometry to advance our understanding of Mycobacterium tuberculosis physiology and pathogenesis. Cytometry Part A, 97(7):683-693. doi:10.1002/cyto.a.24030en_ZA
dc.identifier.issn1552-4930 (online)en_ZA
dc.identifier.otherdoi:10.1002/cyto.a.24030en_ZA
dc.identifier.urihttp://hdl.handle.net/10019.1/125394en_ZA
dc.language.isoen_ZAen_ZA
dc.publisherInternational Society for Advancement of Cytometryen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectMycobacterium tuberculosisen_ZA
dc.subjectBacterial physiologyen_ZA
dc.subjectTuberculosis -- Cytopathologyen_ZA
dc.subjectFlow cytometryen_ZA
dc.subjectHeterogeneityen_ZA
dc.titleRecent developments in the application of flow cytometry to advance our understanding of Mycobacterium tuberculosis physiology and pathogenesisen_ZA
dc.typeArticleen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
parbhoo_recent_2020.pdf
Size:
3.11 MB
Format:
Adobe Portable Document Format
Description:
Download article
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Research Articles (Molecular Biology and Human Genetics)