Point spread function engineering for fluorescence microscopy

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dc.contributor.advisorBosman, Gurthwin W.en_ZA
dc.contributor.advisorRohwer, Erich G.en_ZA
dc.contributor.authorHolinirina Dina Miora, Ratsimandresyen_ZA
dc.contributor.otherStellenbosch University. Faculty of Science. Dept. of Physics.en_ZA
dc.date.accessioned2018-02-26T09:00:02Z
dc.date.accessioned2018-04-09T07:02:59Z
dc.date.available2018-02-26T09:00:02Z
dc.date.available2018-04-09T07:02:59Z
dc.date.issued2018-03
dc.descriptionThesis (MSc)--Stellenbosch University, 2018.en_ZA
dc.description.abstractENGLISH SUMMARY: A tool for biological imaging is developed within this work. It consists of engineering the point spread function (PSF) of a fluorescent molecule or a nanoparticle emitter by modulating the phase of the fluorescence emission. The engineered PSF developed in this current work is called the double helix point spread function (DH-PSF). Information about the three dimensional position of an emitter and its orientation can be extracted using this PSF with a very high precision and accuracy. Other modalities, such as the pyramidal PSF and the bisected PSF, are also simulated and compared theoretically with the efficiency of the DH-PSF.en_ZA
dc.description.abstractAFRIKAANSE OPSOMMING: ‘n Afbeeldingstegniek vir biologiese materie word in hierdie projek ontwikkel. Die tegniek behels die verstelling van die puntverspreidingsfunksie (PVF) van ‘n fluoresserende molekuul of nanopartikel deur die fase van die emissie te moduleer. In die huidige projek word daar gefokus op ‘n spesifieke PVF genaamd, dubbel helix puntverspreidingsfunksie (DH-PVF). Deur die korrekte toepassing van die DH-PVF is dit moontlik om hoë presisie en akkurate drie dimensionele posisie asook orientasie inligting van enkel fluoresserende voorwerpe te bepaal. Verder word daar ook ander modaliteite naamlik piramidale PVF en verdeelde PVF gesimuleer en met die DH-PVF vergelyk.af_ZA
dc.format.extentx, 56 pages ; illustrationsen_ZA
dc.identifier.urihttp://hdl.handle.net/10019.1/103592
dc.language.isoen_ZAen_ZA
dc.publisherStellenbosch : Stellenbosch Universityen_ZA
dc.rights.holderStellenbosch Universityen_ZA
dc.subjectFluorescence microscopyen_ZA
dc.subjectUCTD
dc.subjectDouble Helix Point Spread Functionen_ZA
dc.subjectImaging systemsen_ZA
dc.subjectPhase modulationen_ZA
dc.titlePoint spread function engineering for fluorescence microscopyen_ZA
dc.typeThesisen_ZA
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