Genetic screening of wine-related enzymes in Lactobacillus species isolated from South African wines

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dc.contributor.authorMtshali, P. S.en_ZA
dc.contributor.authorDivol, Benoiten_ZA
dc.contributor.authorVan Rensburg, P.en_ZA
dc.contributor.authorDu Toit, M.en_ZA
dc.date.accessioned2011-05-15T15:59:23Z
dc.date.available2011-05-15T15:59:23Z
dc.date.issued2010
dc.description.abstractAims: The objective of this study was to investigate the presence of genes coding for enzymes of oenological relevance in wine Lactobacillus strains isolated from South African grape and wine samples during the 2001 and 2002 harvest seasons. Methods and Results: A total of 120 wine lactobacilli isolates belonging to Lactobacillus plantarum, Lactobacillus hilgardii, Lactobacillus brevis, Lactobacillus pentosus, Lactobacillus paracasei, Lactobacillus sakei and Lactobacillus paraplantarum were genetically screened for enzyme-encoding genes using PCR with primers specific for β-glucosidase, protease, esterase, citrate lyase and phenolic acid decarboxylase. The results of PCR screening showed that the Lactobacillus strains possessed different combinations of enzymes and that some strains did not possess any of the enzymes tested. Confirmation analysis with gene sequencing also showed high similarity of genes with those available in GenBank database. Conclusion: In this study, we have demonstrated the existence of genes coding for wine-related enzymes in wine lactobacilli that could potentially hydrolyse wine precursors to positively influence wine aroma. Significance and Impact of the Study: An expansion of knowledge on the genetic diversity of wine-associated lactic acid bacteria will enable the selection of novel malolactic fermentation starter cultures with desired oenological traits for the improvement of the organoleptic quality of the wine, and hence wine aroma. © 2009 The Authors.
dc.description.versionArticle
dc.identifier.citationJournal of Applied Microbiology
dc.identifier.citation108
dc.identifier.citation4
dc.identifier.issn13645072
dc.identifier.other10.1111/j.1365-2672.2009.04535.x
dc.identifier.urihttp://hdl.handle.net/10019.1/11149
dc.subjectbeta glucosidase
dc.subjectcarboxylyase
dc.subjectcitric acid
dc.subjectenzyme
dc.subjectesterase
dc.subjectlyase
dc.subjectphenol
dc.subjectprimer DNA
dc.subjectproteinase
dc.subjectbacterium
dc.subjectenzyme
dc.subjectfermentation
dc.subjectgenetic engineering
dc.subjectpolymerase chain reaction
dc.subjecttaxonomy
dc.subjectanalysis
dc.subjectarticle
dc.subjectbacterial strain
dc.subjectbacterium isolate
dc.subjectbacterium isolation
dc.subjectcontrolled study
dc.subjectGenBank
dc.subjectgene sequence
dc.subjectgenetic code
dc.subjectgenetic screening
dc.subjectgrape
dc.subjectharvest period
dc.subjectLactobacillus
dc.subjectLactobacillus brevis
dc.subjectLactobacillus hilgardii
dc.subjectLactobacillus paracasei
dc.subjectLactobacillus pentosus
dc.subjectLactobacillus plantarum
dc.subjectLactobacillus sakei
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectpolymerase chain reaction
dc.subjectsample
dc.subjectscreening
dc.subjectSouth Africa
dc.subjectwine
dc.subjectLactobacillus
dc.subjectLactobacillus brevis
dc.subjectLactobacillus hilgardii
dc.subjectLactobacillus paracasei
dc.subjectLactobacillus paraplantarum
dc.subjectLactobacillus pentosus
dc.subjectLactobacillus plantarum
dc.subjectLactobacillus sakei
dc.subjectVitaceae
dc.titleGenetic screening of wine-related enzymes in Lactobacillus species isolated from South African wines
dc.typeArticle
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