Species identification and comparative population genetics of four coastal houndsharks based on novel NGS-mined microsatellites

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dc.contributor.authorMaduna, Simo N.en_ZA
dc.contributor.authorRossouw, Charneen_ZA
dc.contributor.authorDa Silva, Charleneen_ZA
dc.contributor.authorSoekoe, Michelleen_ZA
dc.contributor.authorBester-Van Der Merwe, Aletta E.en_ZA
dc.date.accessioned2017-02-07T07:23:34Z
dc.date.available2017-02-07T07:23:34Z
dc.date.issued2017
dc.descriptionCITATION: Maduna, S. N., et al. 2017. Species identification and comparative population genetics of four coastal houndsharks based on novel NGS-mined microsatellites. Ecology and Evolution,7(5):1462–1486, doi:10.1002/ece3.2770.en_ZA
dc.descriptionThe original publication is available at http://onlinelibrary.wiley.comen_ZA
dc.description.abstractThe common smooth-hound (Mustelus mustelus) is the topmost bio-economically and recreationally important shark species in southern Africa, western Africa, and Mediterranean Sea. Here, we used the Illumina HiSeq™ 2000 next-generation sequencing (NGS) technology to develop novel microsatellite markers for Mustelus mustelus. Two microsatellite multiplex panels were constructed from 11 polymorphic loci and characterized in two populations of Mustelus mustelus representative of its South African distribution. The markers were then tested for cross-species utility in Galeorhinus galeus, Mustelus palumbes, and Triakis megalopterus, three other demersal coastal sharks also subjected to recreational and/or commercial fishery pressures in South Africa. We assessed genetic diversity (NA, AR, HO, HE, and PIC) and differentiation (FST and Dest) for each species and also examined the potential use of these markers in species assignment. In each of the four species, all 11 microsatellites were variable with up to a mean NA of 8, AR up to 7.5, HE and PIC as high as 0.842. We were able to reject genetic homogeneity for all species investigated here except for T. megalopterus. We found that the panel of the microsatellite markers developed in this study could discriminate between the study species, particularly for those that are morphologically very similar. Our study provides molecular tools to address ecological and evolutionary questions vital to the conservation and management of these locally and globally exploited shark species.en_ZA
dc.description.sponsorshipNational Research Foundation of South Africaen_ZA
dc.description.urihttp://onlinelibrary.wiley.com/doi/10.1002/ece3.2770/abstract
dc.description.versionPublisher’s versionen_ZA
dc.format.extent25 pages : illustrationsen_ZA
dc.identifier.citationMaduna, S. N., et al. 2017. Species identification and comparative population genetics of four coastal houndsharks based on novel NGS-mined microsatellites. Ecology and Evolution, 1–25, doi:10.1002/ece3.2770en_ZA
dc.identifier.issn2045-7758 (online)
dc.identifier.otherdoi:10.1002/ece3.2770
dc.identifier.urihttp://hdl.handle.net/10019.1/100594
dc.language.isoen_ZAen_ZA
dc.publisherWiley Open Accessen_ZA
dc.rights.holderAuthor retains copyrighten_ZA
dc.subjectCross-amplificationen_ZA
dc.subjectShark species -- Southern Africaen_ZA
dc.subjectCommon smooth-hound (Mustelus mustelus)en_ZA
dc.subjectGaleorhinus galeus -- Geneticsen_ZA
dc.subjectMustelus palumbes -- Geneticsen_ZA
dc.subjectTriakis megalopterus -- Geneticsen_ZA
dc.titleSpecies identification and comparative population genetics of four coastal houndsharks based on novel NGS-mined microsatellitesen_ZA
dc.typeArticleen_ZA
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