Modulation of LPS-induced CD4+ T-cell activation and apoptosis by antioxidants in untreated asymptomatic HIV infected participants : an in vitro study

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dc.contributor.authorWanjiku, Samuel Mburu
dc.contributor.authorMarnewick, J. L.
dc.contributor.authorAbayomi, A.
dc.contributor.authorIpp, H.
dc.date.accessioned2014-02-11T09:03:45Z
dc.date.available2014-02-11T09:03:45Z
dc.date.issued2013-11
dc.descriptionPublication of this article was funded by the Stellenbosch University Open Access Fund.en_ZA
dc.descriptionThe original publication is available at http://www.hindawi.com/journals/cdi/en_ZA
dc.description.abstractPersistent immune activation characterises HIV infection and is associated with depletion of CD4+ T-cells and increased risk of disease progression. Early loss of gutmucosal integrity results in the translocation of microbial products such as lipopolysaccharide (LPS) into the systemic circulation. This is an important source of on-going immune stimulation.The purpose of this study was to determine levels of CD4+ T-cell activation (%CD25 expression) and apoptosis (% annexin V/7-AAD) in asymptomatic, untreated HIV infection at baseline and after stimulation with LPS and incubation with or without vitamin C and N-acetylcysteine. LPS induced a significant (𝑃�� < 0.03) increase in %CD25 expression, annexin V, and 7-AAD in HIV positive individuals. NAC in combination with vitamin C, significantly (𝑃�� = 0.0018) reduced activation and early apoptosis of CD4+ T-cells to a greater degree than with either antioxidant alone. Certain combinations of antioxidants could be important in reducing the harmful effects of chronic immune activation and thereby limit CD4+ T-cell depletion. Importantly, we showed that CD4+ T-cells of the HIV positive group responded better to a combination of the antioxidants at this stage than those of the controls.Therefore, appropriate intervention at this asymptomatic stage could rescue the cells before repetitive activation results in the death of CD4+ T-cells.en_ZA
dc.description.sponsorshipStellenbosch Universityen_ZA
dc.description.versionPublishers' versionen_ZA
dc.format.extent8. p : ill
dc.identifier.citationWanjiku, S. M., Marnewick, J. L., Abayomi, A. & Ipp, H. 2013. Modulation of LPS-induced CD4+ T-cell activation and apoptosis by antioxidants in untreated asymptomatic HIV infected participants: An in vitro study. Clinical and Developmental Immunology, 2013:631063, doi:10.1155/2013/631063.en_ZA
dc.identifier.issn1740-2522 (print)
dc.identifier.issn1740-2530 (online)
dc.identifier.otherdoi:10.1155/2013/631063
dc.identifier.urihttp://hdl.handle.net/10019.1/86137
dc.language.isoen_ZAen_ZA
dc.publisherHindawi Publishing Corporationen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectLipopolysaccharide (LPS)en_ZA
dc.subjectInduced stimulationen_ZA
dc.subjectImmunopathogenesisen_ZA
dc.subjectCD4+T cells
dc.subjectImmune activation and HIV patientsen_ZA
dc.subjectAntioxidants and HIV patientsen_ZA
dc.titleModulation of LPS-induced CD4+ T-cell activation and apoptosis by antioxidants in untreated asymptomatic HIV infected participants : an in vitro studyen_ZA
dc.typeArticleen_ZA
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