Detection of a combination of serum IgG and IgA antibodies against selected mycobacterial targets provides promising diagnostic signatures for active TB
Date
2017-03-24
Journal Title
Journal ISSN
Volume Title
Publisher
Impact Journals
Abstract
ENGLISH ABSTRACT: Immunoglobulin G (IgG) based tests for the diagnosis of active tuberculosis (TB)
disease often show a lack of specificity in TB endemic regions, which is mainly due to a
high background prevalence of LTBI. Here, we investigated the combined performance
of the responses of different Ig classes to selected mycobacterial antigens in primary
healthcare clinic attendees with signs and symptoms suggestive of TB. The sensitivity
and specificity of IgA, IgG and/or IgM to LAM and 7 mycobacterial protein antigens
(ESAT-6, Tpx, PstS1, AlaDH, MPT64, 16kDa and 19kDa) and 2 antigen combinations
(TUB, TB-LTBI) in the plasma of 63 individuals who underwent diagnostic work-up
for TB after presenting with symptoms and signs compatible with possible active
TB were evaluated. Active TB was excluded in 42 individuals of whom 21 has LTBI
whereas active TB was confirmed in 21 patients of whom 19 had a follow-up blood
draw at the end of 6-month anti-TB treatment. The leading single serodiagnostic
markers to differentiate between the presence or absence of active TB were anti-16
kDa IgA, anti-MPT64 IgA with sensitivity and specificity of 90%/90% and 95%/90%,
respectively. The combined use of 3 or 4 antibodies further improved this performance
to accuracies above 95%. After successful completion of anti-TB treatment at month
6, the levels of 16 kDa IgA and 16 kDa IgM dropped significantly whereas LAM IgG and
TB-LTBI IgG increased. These results show the potential of extending investigation
of anti-tuberculous IgG responses to include IgM and IgA responses against selected
protein and non-protein antigens in differentiating active TB from other respiratory
diseases in TB endemic settings.
Description
CITATION: Awoniyi, D. O., et al. 2017. Detection of a combination of serum IgG and IgA antibodies against selected mycobacterial targets provides promising diagnostic signatures for active TB. Oncotarget, 8(23):37525-37537, doi:10.18632/oncotarget.16401.
The original publication is available at http://www.oncotarget.com
The original publication is available at http://www.oncotarget.com
Keywords
Antibodies, Tuberculosis -- Diagnosis, Biochemical markers, Mycobacterial diseases
Citation
Awoniyi, D. O., et al. 2017. Detection of a combination of serum IgG and IgA antibodies against selected mycobacterial targets provides promising diagnostic signatures for active TB. Oncotarget, 8(23):37525-37537, doi:10.18632/oncotarget.16401