Linking gene regulation and the exo-metabolome : a comparative transcriptomics approach to identify genes that impact on the production of volatile aroma compounds in yeast

Rossouw, Debra; Naes, Tormod; Bauer, Florian F.

Linking gene regulation and the exo-metabolome : a comparative transcriptomics approach to identify genes that impact on the production of volatile aroma compounds in yeast

dc.contributor.author	Rossouw, Debra
dc.contributor.author	Naes, Tormod
dc.contributor.author	Bauer, Florian F.
dc.date.accessioned	2010-12-13T07:52:22Z
dc.date.available	2010-12-13T07:52:22Z
dc.date.issued	2008-11
dc.date.updated	2010-11-03T12:30:13Z
dc.description.abstract	Background: 'Omics' tools provide novel opportunities for system-wide analysis of complex cellular functions. Secondary metabolism is an example of a complex network of biochemical pathways, which, although well mapped from a biochemical point of view, is not well understood with regards to its physiological roles and genetic and biochemical regulation. Many of the metabolites produced by this network such as higher alcohols and esters are significant aroma impact compounds in fermentation products, and different yeast strains are known to produce highly divergent aroma profiles. Here, we investigated whether we can predict the impact of specific genes of known or unknown function on this metabolic network by combining whole transcriptome and partial exo-metabolome analysis. Results: For this purpose, the gene expression levels of five different industrial wine yeast strains that produce divergent aroma profiles were established at three different time points of alcoholic fermentation in synthetic wine must. A matrix of gene expression data was generated and integrated with the concentrations of volatile aroma compounds measured at the same time points. This relatively unbiased approach to the study of volatile aroma compounds enabled us to identify candidate genes for aroma profile modification. Five of these genes, namely YMR210W, BAT1, AAD10, AAD14 and ACS1 were selected for over expression in commercial wine yeast, VIN13. Analysis of the data show a statistically significant correlation between the changes in the exo-metabome of the over expressing strains and the changes that were predicted based on the unbiased alignment of transcriptomic and exo-metabolomic data. Conclusion: The data suggest that a comparative transcriptomics and metabolomics approach can be used to identify the metabolic impacts of the expression of individual genes in complex systems, and the amenability of transcriptomic data to direct applications of biotechnological relevance.	en_ZA
dc.description.version	Peer Reviewed
dc.identifier.citation	Rossouw, D, Næs, T & Bauer, FF 2008, 'Linking gene regulation and the exo-metabolome: A comparative transcriptomics approach to identify genes that impact on the production of volatile aroma compounds in yeast', BMC Genomics, 9(1):530, http://www.biomedcentral.com/1471-2164/9/530.	en_ZA
dc.identifier.issn	1471-2164
dc.identifier.other	http://dx.doi.org/10.1186/1471-2164-9-530
dc.identifier.uri	http://hdl.handle.net/10019.1/5052
dc.language.iso	en_ZA	en_ZA
dc.language.rfc3066	en
dc.publisher	BioMed Central	en_ZA
dc.rights.holder	Rossouw et al.; licensee BioMed Central Ltd.	en_ZA
dc.subject	Commercial wine yeast strains	en_ZA
dc.subject	Fermentation kinetics and metabolite formation	en_ZA
dc.subject	Microarray analysis	en_ZA
dc.subject	Multivariate analysis of metabolite concentrations and gene expression data	en_ZA
dc.subject	Overexpression of selected genes	en_ZA
dc.title	Linking gene regulation and the exo-metabolome : a comparative transcriptomics approach to identify genes that impact on the production of volatile aroma compounds in yeast	en_ZA
dc.type	Article	en_ZA