Comparison of quantitative techniques including Xpert MTB/RIF to evaluate mycobacterial burden

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dc.contributor.authorVan Zyl-Smit, Richard N.en_ZA
dc.contributor.authorBinder, Ankeen_ZA
dc.contributor.authorMeldau, Richarden_ZA
dc.contributor.authorMishra, H.en_ZA
dc.contributor.authorSemple, P. L.en_ZA
dc.contributor.authorTheron, G.en_ZA
dc.contributor.authorPeter, J.en_ZA
dc.contributor.authorWhitelaw, A.en_ZA
dc.contributor.authorSharma, S. K.en_ZA
dc.contributor.authorWarren, Roben_ZA
dc.contributor.authorBateman, E. D.en_ZA
dc.contributor.authorDheda, K.en_ZA
dc.date.accessioned2013-03-15T08:27:08Z
dc.date.available2013-03-15T08:27:08Z
dc.date.issued2011-12
dc.descriptionThe original publication is available at www.plosone.orgen_ZA
dc.description.abstractIntroduction: Accurate quantification of mycobacterial load is important for the evaluation of patient infectiousness, disease severity and monitoring treatment response in human and in-vitro laboratory models of disease. We hypothesized that newer techniques would perform as well as solid media culture to quantify mycobacterial burden in laboratory specimens. Methods: We compared the turn-around-time, detection-threshold, dynamic range, reproducibility, relative discriminative ability, of 4 mycobacterial load determination techniques: automated liquid culture (BACTEC-MGIT-960), [3H]-uracil incorporation assays, luciferase-reporter construct bioluminescence, and quantitative PCR(Xpert -MTB/RIF) using serial dilutions of Mycobacterium bovis and Mycobacterium tuberculosis H37RV. Mycobacterial colony-forming-units(CFU) using 7H10-Middlebrook solid media served as the reference standard. Results: All 4 assays correlated well with the reference standard, however, bioluminescence and uracil assays had a detection threshold $16103 organisms. By contrast, BACTEC-MGIT-960 liquid culture, although only providing results in days, was user-friendly, had the lowest detection threshold (,10 organisms), the greatest discriminative ability (1 vs. 10 organisms; p = 0.02), and the best reproducibility (coefficient of variance of 2% vs. 38% compared to uracil incorporation; p = 0.02). Xpert-MTB/RIF correlated well with mycobacterial load, had a rapid turn-around-time (,2 hours), was user friendly, but had a detection limit of ,100 organisms. Conclusions: Choosing a technique to quantify mycobacterial burden for laboratory or clinical research depends on availability of resources and the question being addressed. Automated liquid culture has good discriminative ability and low detection threshold but results are only obtained in days. Xpert MTB/RIF provides rapid quantification of mycobacterial burden, but has a poorer discrimination and detection threshold.en_ZA
dc.format.extent10 p. : ill.
dc.identifier.citationVan Zyl-Smit, R. N. et al. 2011. Comparison of Quantitative Techniques including Xpert MTB/RIF to Evaluate Mycobacterial Burden. PLoS ONE, 6(12): e28815, doi:10.1371/journal.pone.0028815.en_ZA
dc.identifier.issn1932-6203 (online)
dc.identifier.otherdoi:10.1371/journal.pone.0028815
dc.identifier.urihttp://hdl.handle.net/10019.1/80405
dc.language.isoen_ZAen_ZA
dc.publisherPLOSen_ZA
dc.rights.holderAuthors retain copyrigten_ZA
dc.subjectMycobacterial loaden_ZA
dc.subjectPatient infectiousnessen_ZA
dc.subjectMycobacterium tuberculosisen_ZA
dc.titleComparison of quantitative techniques including Xpert MTB/RIF to evaluate mycobacterial burdenen_ZA
dc.typeArticleen_ZA
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