Antibacterial, antioxidant and tyrosinase-inhibition activities of pomegranate fruit peel methanolic extract

Simple item page
dc.contributor.authorFawole, Olaniyi A.
dc.contributor.authorMakunga, Nokwanda P.
dc.contributor.authorOpara, Umezuruike L.
dc.date.accessioned2013-05-07T09:52:16Z
dc.date.available2013-05-07T09:52:16Z
dc.date.issued2012-10
dc.date.updated2012-12-21T06:44:33Z
dc.descriptionThe original publication is available at http://www.biomedcentral.com/1472-6882/12/200en_ZA
dc.descriptionPublication of this article was funded by the Stellenbosch University Open Access Fund.
dc.description.abstractAbstract Background This study evaluated, using in vitro assays, the antibacterial, antioxidant, and tyrosinase-inhibition activities of methanolic extracts from peels of seven commercially grown pomegranate cultivars. Methods Antibacterial activity was tested on Gram-positive (Bacillus subtilis and Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli and Klebsiella pneumonia) using a microdilution method. Several potential antioxidant activities, including radical-scavenging ability (RSA), ferrous ion chelating (FIC) and ferric ion reducing antioxidant power (FRAP), were evaluated. Tyrosinase enzyme inhibition was investigated against monophenolase (tyrosine) and diphenolase (DOPA), with arbutin and kojic acid as positive controls. Furthermore, phenolic contents including total flavonoid content (TFC), gallotannin content (GTC) and total anthocyanin content (TAC) were determined using colourimetric methods. HPLC-ESI/MSn analysis of phenolic composition of methanolic extracts was also performed. Results Methanolic peel extracts showed strong broad-spectrum activity against Gram-positive and Gram-negative bacteria, with the minimum inhibitory concentrations (MIC) ranging from 0.2 to 0.78 mg/ml. At the highest concentration tested (1000 μg/ml), radical scavenging activities were significantly higher in Arakta (83.54%), Ganesh (83.56%), and Ruby (83.34%) cultivars (P< 0.05). Dose dependent FIC and FRAP activities were exhibited by all the peel extracts. All extracts also exhibited high inhibition (>50%) against monophenolase and diphenolase activities at the highest screening concentration. The most active peel extract was the Bhagwa cultivar against monophenolase and the Arakta cultivar against diphenolase with IC50 values of 3.66 μg/ml and 15.88 μg/ml, respectively. High amounts of phenolic compounds were found in peel extracts with the highest and lowest total phenolic contents of 295.5 (Ganesh) and 179.3 mg/g dry extract (Molla de Elche), respectively. Catechin, epicatechin, ellagic acid and gallic acid were found in all cultivars, of which ellagic acid was the most abundant comprising of more than 50% of total phenolic compounds detected in each cultivar. Conclusions The present study showed that the tested pomegranate peels exhibited strong antibacterial, antioxidant and tyrosinase-inhibition activities. These results suggest that pomegranate fruit peel could be exploited as a potential source of natural antimicrobial and antioxidant agents as well as tyrosinase inhibitors.en_ZA
dc.description.versionPublishers' Versionen_ZA
dc.format.extent11 p. : ill.
dc.identifier.citationFawole, O. A., Makunga, N. P. & Opara, U. l. 2012. Antibacterial, antioxidant and tyrosinase-inhibition activities of pomegranate fruit peel methanolic extract. BMC Complementary and Alternative Medicine, 12(1):200, doi.org/10.1186/1472-6882-12-200.en_ZA
dc.identifier.issn1472-6882 (online)
dc.identifier.urihttp://dx.doi.org/10.1186/1472-6882-12-200
dc.identifier.urihttp://hdl.handle.net/10019.1/80723
dc.language.isoen_ZAen_ZA
dc.language.rfc3066en
dc.publisherBioMed Centralen_ZA
dc.rights.holderOlaniyi A Fawole et al.; licensee BioMed Central Ltd.en_ZA
dc.subjectPomegranate (Punica granatum L.; Punicaceae)en_ZA
dc.subjectPomegranate peel extract -- Nutritional valueen_ZA
dc.titleAntibacterial, antioxidant and tyrosinase-inhibition activities of pomegranate fruit peel methanolic extracten_ZA
dc.typeArticleen_ZA
Files
Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
1472-6882-12-200.xml
Size:
111.52 KB
Format:
Extensible Markup Language
Description:
Download
Thumbnail Image
Name:
1472-6882-12-200.pdf
Size:
569.69 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.95 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Research Articles (Botany and Zoology)