Detoxification of the fumonisin mycotoxins in maize : an enzymatic approach

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dc.contributor.authorAlberts, Johannaen_ZA
dc.contributor.authorSchatzmayr, Gerden_ZA
dc.contributor.authorMoll, Wulf-Dieteren_ZA
dc.contributor.authorDavids, Ibtisaamen_ZA
dc.contributor.authorRheeder, Johnen_ZA
dc.contributor.authorBurger, Hester-Marien_ZA
dc.contributor.authorShephard, Gordonen_ZA
dc.contributor.authorGelderblom, Wentzelen_ZA
dc.date.accessioned2021-01-28T09:31:30Z
dc.date.available2021-01-28T09:31:30Z
dc.date.issued2019-09-10
dc.descriptionCITATION: Alberts, J., et al. 2019. Detoxification of the fumonisin mycotoxins in maize : an enzymatic approach. Toxins, 11(9):523, doi:10.3390/toxins11090523.
dc.descriptionThe original publication is available at https://www.mdpi.com
dc.description.abstractEnzymatic detoxification has become a promising approach for control of mycotoxins postharvest in grains through modification of chemical structures determining their toxicity. In the present study fumonisin esterase FumD (EC 3.1.1.87) (FUMzyme®; BIOMIN, Tulln, Austria), hydrolysing fumonisin (FB) mycotoxins by de-esterification, was utilised to develop an enzymatic reduction method in a maize kernel enzyme incubation mixture. Efficacy of the FumD FB reduction method in “low” and “high” FB contaminated home-grown maize was compared by monitoring FB1 hydrolysis to the hydrolysed FB1 (HFB1) product utilising a validated LC-MS/MS analytical method. The method was further evaluated in terms of enzyme activity and treatment duration by assessing enzyme kinetic parameters and the relative distribution of HFB1 between maize kernels and the residual aqueous environment. FumD treatments resulted in significant reduction (≥80%) in “low” (≥1000 U/L, p < 0.05) and “high” (100 U/L, p < 0.05; ≥1000 U/L, p < 0.0001) FB contaminated maize after 1 h respectively, with an approximate 1:1 µmol conversion ratio of FB1 into the formation of HFB1. Enzyme kinetic parameters indicated that, depending on the activity of FumD utilised, a significantly (p < 0.05) higher FB1 conversion rate was noticed in “high” FB contaminated maize. The FumD FB reduction method in maize could find application in commercial maize-based practices as well as in communities utilising home-grown maize as a main dietary staple and known to be exposed above the tolerable daily intake levels.en_ZA
dc.description.urihttps://www.mdpi.com/2072-6651/11/9/523
dc.description.versionPublisher's version
dc.format.extent15 pages
dc.identifier.citationAlberts, J., et al. 2019. Detoxification of the fumonisin mycotoxins in maize : an enzymatic approach. Toxins, 11(9):523, doi:10.3390/toxins11090523
dc.identifier.issn2072-6651 (online)
dc.identifier.otherdoi:10.3390/toxins11090523
dc.identifier.urihttp://hdl.handle.net/10019.1/109032
dc.language.isoen_ZAen_ZA
dc.publisherMDPI
dc.rights.holderAuthors retain copyright
dc.subjectFumonisins -- Analysisen_ZA
dc.subjectEnzymatic detoxificationen_ZA
dc.subjectMycotoxinsen_ZA
dc.subjectField crops -- Diseases and pests -- Controlen_ZA
dc.subjectCorn as food -- Analysisen_ZA
dc.subjectPlants -- Effect of mycotoxins onen_ZA
dc.titleDetoxification of the fumonisin mycotoxins in maize : an enzymatic approachen_ZA
dc.typeArticleen_ZA
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