In vitro influence of sublethal hypoxia on differentiation of the 3T3-L1 preadipose cell line and its physiological implications
Date
1997
Authors
Macfarlane C.M.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Exposure of cultures of 3T3-L1 preadipose cells to nitrogen for 16 hours kills almost all of the cells, but after exposure to 5% oxygen for 16 hours most of the cells survive, and recover when culture is continued in 20% oxygen. The extent of recovery depends on the insulin concentration of the medium. Isotope incorporation and flow cytometry experiments show that exposure to 5% oxygen for 16 hours growth arrests the cells and leads to an elongation of the G1-phase of the cell cycle. When 3T3-L1 cells are growth arrested in the presence of 5% oxygen and allowed to recover in the presence of 5μg/ml insulin under 20% oxygen, they can be induced to differentiate by treatment with carbacyclin during the period of growth arrest. Activity of the marker enzyme glycerol-3-phosphate dehydrogenase increases from 46.5±17 mU/mg protein to 1506±271 mU/mg protein. The extent of differentiation is exponentially related to the concentration of carbacyclin in the medium.
Description
Keywords
adipocyte, animal, article, cell differentiation, cell division, cell hypoxia, cell strain 3T3, cell strain HL 60, culture medium, cytology, electron microscopy, flow cytometry, human, mouse, physiology, ultrastructure, 3T3 Cells, Adipocytes, Animals, Cell Differentiation, Cell Division, Cell Hypoxia, Culture Media, Flow Cytometry, HL-60 Cells, Humans, Mice, Microscopy, Electron
Citation
Life Sciences
60
21
60
21