The kinetics of the humoral and interferon-gamma immune responses to experimental Mycobacterium bovis infection in the white rhinoceros (Ceratotherium simum)

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dc.contributor.authorParsons, Sven D. C.en_ZA
dc.contributor.authorMorar-Leather, Darshanaen_ZA
dc.contributor.authorBuss, Peter E.en_ZA
dc.contributor.authorHofmeyr, Jenniferen_ZA
dc.contributor.authorMcFadyen, Rossen_ZA
dc.contributor.authorRutten, Victor P. M. G.en_ZA
dc.contributor.authorVan Helden, Paul D.en_ZA
dc.contributor.authorMiller, Michele Annen_ZA
dc.contributor.authorMichel, Anita Luiseen_ZA
dc.date.accessioned2018-10-09T10:28:42Z
dc.date.available2018-10-09T10:28:42Z
dc.date.issued2017-12
dc.descriptionCITATION: Parsons, S. D. C. et al. 2017. The kinetics of the humoral and interferon-gamma immune responses to experimental Mycobacterium bovis infection in the white rhinoceros (Ceratotherium simum). Frontiers in Immunology, 8:1831, doi:10.3389/fimmu.2017.01831.
dc.descriptionThe original publication is available at https://www.frontiersin.org/journals/immunology
dc.description.abstractENGLISH ABSTRACT: Mycobacterium bovis is the cause of tuberculosis (TB) in a wide range of species, including white rhinoceroses (Ceratotherium simum). Control of the disease relies on the indirect detection of infection by measuring pathogen-specific responses of the host. These are poorly described in the white rhinoceros and this study aimed to characterize the kinetics of immune responses to M. bovis infection in this species. Three white rhinoceroses were infected with M. bovis and their immune sensitization to this pathogen was measured monthly for 20 months. Cell-mediated immunity was characterized in whole blood samples as the differential release of interferon-gamma in response to bovine purified protein derivative (PPDb) and avian PPD (PPDa) as well as the release of this cytokine in response to the M. bovis proteins 6 kDa early secretory antigenic target (ESAT-6)/10 kDa culture filtrate protein (CFP-10). Humoral immunity was quantified as the occurrence or the magnitude of antibody responses to the proteins ESAT-6/CFP-10, MPB83, MPB83/MPB70, and PPDb. The magnitude and duration of immune reactivity varied between individuals; however, peak responses to these antigens were detected in all animals circa 5–9 months postinfection. Hereafter, they gradually declined to low or undetectable levels. This pattern was associated with limited TB-like pathology at postmortem examination and appeared to reflect the control of M. bovis infection following the development of the adaptive immune response. Measurement of these markers could prove useful for assessing the disease status or treatment of naturally infected animals. Moreover, immune responses identified in this study might be used to detect infection; however, further studies are required to confirm their diagnostic utility.en_ZA
dc.description.sponsorshipSouth African Medical Research Council (SAMRC)
dc.description.sponsorshipSouth African National Research Foundation (NRF)
dc.description.urihttps://www.frontiersin.org/articles/10.3389/fimmu.2017.01831/full
dc.description.versionPublisher's version
dc.format.extent7 pagesen_ZA
dc.identifier.citationParsons, S. D. C. et al. 2017. The kinetics of the humoral and interferon-gamma immune responses to experimental Mycobacterium bovis infection in the white rhinoceros (Ceratotherium simum). Frontiers in Immunology, 8:1831, doi:10.3389/fimmu.2017.01831.
dc.identifier.issn1664-3224 (online)
dc.identifier.otherdoi:10.3389/fimmu.2017.01831
dc.identifier.urihttp://hdl.handle.net/10019.1/104551
dc.language.isoen_ZAen_ZA
dc.publisherFrontiersen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectTuberculosisen_ZA
dc.subjectWhite rhinocerosen_ZA
dc.subjectMycobacterial diseasesen_ZA
dc.titleThe kinetics of the humoral and interferon-gamma immune responses to experimental Mycobacterium bovis infection in the white rhinoceros (Ceratotherium simum)en_ZA
dc.typeArticleen_ZA
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