The effect on cleavage of two-cell mouse embryos after a delay in embryo retrieval in a human in vitro fertilization programme

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dc.contributor.authorKruger, T. F.en_ZA
dc.contributor.authorStander, F. S. H.en_ZA
dc.date.accessioned2011-03-18T14:57:33Z
dc.date.available2011-03-18T14:57:33Z
dc.date.issued1985
dc.descriptionCITATION: Kruger, T. F. & Stander, F. S. H. The effect on cleavage of two-cell mouse embryos after a delay in embryo retrieval in a human in vitro fertilization programme. South African Medical Journal, 68:743-744.
dc.descriptionThe original publication is available at http://www.samj.org.za
dc.description.abstractTwo-cell mouse embryos are used for quality control in a human in vitro fertilization programme. A controlled experiment was designed to evaluate the effect on cleavage of two-cell mouse embryos after a delay in embryo retrieval. In the test group, two fallopian tubes were incubated in Whittingham's T6 medium for 2 1/2 hours per experiment before the embryos were removed for culture. In the control group embryos were removed from the fallopian tubes immediately after the mice were sacrificed. Five experiments were performed. Eight of 141 two-cell embryos (5,7%) reached the balstocyst stage in the test group, and in the control group 143 of 151 two-cell embryos (94,7%) reached the blastocyst stage after 72 hours. Embryos must be removed immediately after the mice are sacrificed to obtain constant results. If not, poor cleavage can lead to unnecessary confusion in the laboratory.
dc.description.versionPublisher’s version
dc.identifier.issn2078-5135 (online)
dc.identifier.issn0256-9574 (print)
dc.identifier.urihttp://hdl.handle.net/10019.1/7498
dc.language.isoen
dc.publisherHealth & Medical Publishing Group
dc.rights.holderSouth African Medical Journal
dc.subjectFertilization in vitroen_ZA
dc.titleThe effect on cleavage of two-cell mouse embryos after a delay in embryo retrieval in a human in vitro fertilization programmeen_ZA
dc.typeArticle
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