Lactobacillus equigenerosi strain Le1 invades equine epithelial cells

Simple item page
dc.contributor.authorBotha, Marlieen_ZA
dc.contributor.authorBotes, Marelizeen_ZA
dc.contributor.authorLoos, Benen_ZA
dc.contributor.authorSmith, Carineen_ZA
dc.contributor.authorDicks, Leon Milner Theodore, 1961-en_ZA
dc.date.accessioned2012-08-02T09:13:52Z
dc.date.available2012-08-02T09:13:52Z
dc.date.issued2012
dc.descriptionCITATION: Botha, M., et al. 2012. Lactobacillus equigenerosi strain Le1 invades equine epithelial cells. Applied and Environmental Microbiology, 78(12):4248-4255, doi:10.1128/AEM.00552-12.
dc.descriptionThe original publication is available at https://aem.asm.org
dc.description.abstractLactobacillus equigenerosi strain Le1, a natural inhabitant of the equine gastrointestinal tract, survived pH 3.0 and incubation in the presence of 1.5% (wt/vol) bile salts for at least 2 h. Strain Le1 showed 8% cell surface hydrophobicity, 60% auto-aggregation, and 47% coaggregation with Clostridium difficile C6. Only 1% of the cells adhered to viable buccal epithelial cells and invaded the cells within 20 min after contact. Preincubation of strain Le1 in a buffer containing pronase prevented adhesion to viable epithelial cells. Preincubation in a pepsin buffer delayed invasion from 20 min to 1 h. Strain Le1 did not adhere to nonviable epithelial cells. Administration of L. equigenerosi Le1 (1x10 9 CFU per 50 kg body weight) to healthy horses did not increase white blood cell numbers. Differential white blood cell counts and aspartate aminotransferase levels remained constant. Glucose, lactate, cholesterol, and urea levels remained constant during administration with L. equigenerosi Le1 but decreased during the week after administration.
dc.description.urihttps://aem.asm.org/content/78/12/4248
dc.description.versionPublisher's version
dc.format.extent9 pages
dc.identifier.citationBotha, M., et al. 2012. Lactobacillus equigenerosi strain Le1 invades equine epithelial cells. Applied and Environmental Microbiology, 78(12):4248-4255, doi:10.1128/AEM.00552-12
dc.identifier.issn1098-5336 (online)
dc.identifier.issn0099-2240 (print)
dc.identifier.otherdoi:10.1128/AEM.00552-12
dc.identifier.urihttp://hdl.handle.net/10019.1/21947
dc.language.isoen_ZAen_ZA
dc.publisherAmerican Society for Microbiology
dc.rights.holderAmerican Society for Microbiology
dc.subjectLactobacillus
dc.subjectEpithelial cells
dc.subjectHorses
dc.titleLactobacillus equigenerosi strain Le1 invades equine epithelial cellsen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
botha_lactobacillus_2012.pdf
Size:
1.18 MB
Format:
Adobe Portable Document Format
Description:
Download article
Download
Collections
Research Articles (Microbiology)