DNA vaccines against mycoplasma elicit humoral immune responses in ostriches
Date
2019-05-14
Journal Title
Journal ISSN
Volume Title
Publisher
Frontiers Media
Abstract
In ostriches, the population densities resulting from intensive rearing increases
susceptibility to pathogens such as mycoplasmas. In addition to good management
practices, vaccination offers an attractive alternative for controlling mycoplasma
infections in food animals, instead of using antibiotics, which often leave unacceptable
residues. The use of live attenuated vaccines, however, carry the concern of reversion to
virulence or genetic recombination with field strains. Currently there are no commercially
available vaccines against ostrich-infecting mycoplasmas and this study therefore set
out to develop and evaluate the use of a DNA vaccine against mycoplasma infections in
ostriches using an OppA protein as antigen. To this end, the oppA gene of “Mycoplasma
nasistruthionis sp. nov.” str. Ms03 was cloned into two DNA vaccine expression vectors
after codon correction by site-directed mutagenesis. Three-months-old ostriches were
then vaccinated intramuscularly at different doses followed by a booster vaccination
after 6 weeks. The ability of the DNA vaccines to elicit an anti-OppA antibody response
was evaluated by ELISA using the recombinant OppA protein of Ms03 as coating
antigen. A statistically significant anti-OppA antibody response could be detected after
administration of a booster vaccination indicating that the OppA protein was successfully
immunogenic. The responses were also both dose and vector dependent. In conclusion,
the DNA vaccines were able to elicit an immune response in ostriches and can therefore
be viewed as an option for the development of vaccines against mycoplasma infections.
Description
The original publicaiton is available from https://www.frontiersin.org/journals/immunology#
CITATION: Wium, M. et al. 2019. DNA vaccines against Mycoplasma elicit humoral immune responses in ostriches. Frontiers in Immunology, 10:1061, doi:10.3389/fimmu.2019.01061.
CITATION: Wium, M. et al. 2019. DNA vaccines against Mycoplasma elicit humoral immune responses in ostriches. Frontiers in Immunology, 10:1061, doi:10.3389/fimmu.2019.01061.
Keywords
Ostriches -- Diseases -- Treatment, Ostrich-infecting mycoplasmas, DNA vaccines, OppA protein as antigen, Antibody response, Mycoplasma diseases -- Vaccination
Citation
Wium, M. et al. 2019. DNA vaccines against Mycoplasma elicit humoral immune responses in ostriches. Frontiers in Immunology, 10:1061, doi:10.3389/fimmu.2019.01061.