Comparison of cross-sectional HIV incidence assay results from dried blood spots and plasma

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dc.contributor.authorSchlusser, Katherine E.en_ZA
dc.contributor.authorPilcher, Christopheren_ZA
dc.contributor.authorKallas, Esper G.en_ZA
dc.contributor.authorSantos, Breno R.en_ZA
dc.contributor.authorDeeks, Steven G.en_ZA
dc.contributor.authorFacente, Shelleyen_ZA
dc.contributor.authorKeating, Sheila M.en_ZA
dc.contributor.authorBusch, Michael P.en_ZA
dc.contributor.authorMurphy, Garyen_ZA
dc.contributor.authorWelte, Alexen_ZA
dc.contributor.authorQuinn, Thomasen_ZA
dc.contributor.authorEshleman, Susan H.en_ZA
dc.contributor.authorLaeyendecker, Oliveren_ZA
dc.date.accessioned2017-03-15T09:27:55Z
dc.date.available2017-03-15T09:27:55Z
dc.date.issued2017-02-23
dc.descriptionCITATION: Schlusser, K. E. et al. 2017. Comparison of cross-sectional HIV incidence assay results from dried blood spots and plasma. PLoS ONE, E 12(2):e0172283, doi:10.1371/journal.pone.0172283.
dc.descriptionThe original publication is available at http://journals.plos.org/plosone
dc.description.abstractBackground: Assays have been developed for cross-sectional HIV incidence estimation using plasma samples. Large scale surveillance programs are planned using dried blood spot (DBS) specimens for incidence assessment. However, limited information exists on the performance of HIV cross-sectional incidence assays using DBS. Methods: The assays evaluated were: Maxim HIV-1 Limiting Antigen Avidity EIA (LAg-Avidity), Sedia HIV-1 BED-Capture EIA (BED-CEIA), and CDC modified BioRad HIV-1/2 Plus O Avidity-based Assay (CDC-BioRad Avidity) using pre-determined cutoff values. 100 matched HIV-1 positive plasma and DBS samples, with known duration of infection, from the Consortium for the Evaluation and Performance of HIV Incidence Assays repository were tested. All assays were run in duplicate. To examine the degree of variability within and between results for each sample type, both categorical and continuous results were analyzed. Associations were assessed with Bland Altman, R2 values and Cohen’s kappa coefficient (ĸ). Results: Intra-assay variability using the same sample type was similar for all assays (R2 0.96 to 1.00). The R2 values comparing DBS and plasma results for LAg-Avidity, BED-CEIA, and CDC-BioRad Avidity were 0.96, 0.94, and 0.84, respectively. The concordance and ĸ values between DBS and plasma for all three assays were >87% and >0.64, respectively. The Bland-Altman analysis showed significant differences between plasma and DBS samples. For all three assays, a higher number of samples were classified as recent infections using DBS samples. Conclusions: DBS and plasma sample results were highly correlated. However, when compared to plasma, each assay performed somewhat differently in DBS at the lower and higher ends of the dynamic range. DBS samples were more likely to be classified as recently infected by all three assays, which may lead to overestimation of incidence in surveys using performance criteria derived for plasma samples.en_ZA
dc.description.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0172283
dc.description.versionPublisher's version
dc.format.extent10 pages
dc.identifier.citationSchlusser, K. E. et al. 2017. Comparison of cross-sectional HIV incidence assay results from dried blood spots and plasma. PLoS ONE, E 12(2):e0172283, doi:10.1371/journal.pone.0172283.
dc.identifier.issn1932-6203 (online)
dc.identifier.otherdoi:10.1371/journal.pone.0172283
dc.identifier.urihttp://hdl.handle.net/10019.1/100678
dc.language.isoen_ZAen_ZA
dc.publisherPublic Library of Science
dc.rights.holderAuthors retain copyright
dc.subjectHIV infections -- Diagnosisen_ZA
dc.subjectPlasma (Blood) samples -- Testingen_ZA
dc.subjectDried blood spots -- Testingen_ZA
dc.titleComparison of cross-sectional HIV incidence assay results from dried blood spots and plasmaen_ZA
dc.typeArticleen_ZA
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