Redox status and muscle pathology in rheumatoid arthritis : insights from various rat hindlimb muscles
Date
2019-03-26
Journal Title
Journal ISSN
Volume Title
Publisher
Hindawi
Abstract
Due to atrophy, muscle weakness is a common occurrence in rheumatoid arthritis (RA). The majority of human studies are
conducted on the vastus lateralis muscle—a muscle with mixed fiber type—but little comparative data between multiple muscles
in either rodent or human models are available. The current study therefore assessed both muscle ultrastructure and selected
redox indicators across various muscles in a model of collagen-induced rheumatoid arthritis in female Sprague-Dawley rats.
Only three muscles, the gastrocnemius, extensor digitorum longus (EDL), and soleus, had lower muscle mass (38%, 27%, and 25%
loss of muscle mass, respectively; all at least P < 0. 01), while the vastus lateralis muscle mass was increased by 35% (P < 0. 01) in
RA animals when compared to non-RA controls. However, all four muscles exhibited signs of deterioration indicative of
rheumatoid cachexia. Cross-sectional area was similarly reduced in gastrocnemius, EDL, and soleus (60%, 58%, and 64%,
respectively; all P < 0. 001), but vastus lateralis (22% smaller, P < 0. 05) was less affected, while collagen deposition was
significantly increased in muscles. This pathology was associated with significant increases in tissue levels of reactive oxygen
species (ROS) in all muscles except the vastus lateralis, while only the gastrocnemius had significantly increased levels of lipid
peroxidation (TBARS) and antioxidant activity (FRAP). Current data illustrates the differential responses of different skeletal
muscles of the hindlimb to a chronic inflammatory challenge both in terms of redox changes and resistance to cachexia.
Description
CITATION: Oyenihi, A. B., et al. 2019. Redox status and muscle pathology in rheumatoid arthritis : insights from various rat hindlimb muscles. Oxidative Medicine and Cellular Longevity, 2019, Article ID 2484678, doi:10.1155/2019/2484678.
Publication of this article was funded by the Stellenbosch University Open Access Fund
Publication of this article was funded by the Stellenbosch University Open Access Fund
Keywords
Muscle weakness, Rheumatoid arthritis, Muscle pathology in rheumatoid arthritis, Redox status in rheumatoid arthritis
Citation
Oyenihi, A. B., et al. 2019. Redox status and muscle pathology in rheumatoid arthritis : insights from various rat hindlimb muscles. Oxidative Medicine and Cellular Longevity, 2019, Article ID 2484678, doi:10.1155/2019/2484678