An evaluation of the diagnostic adequacy and immunocytochemistry of manual liquid-based smears in breast aspirates

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dc.contributor.authorShibemba, A. L.en_ZA
dc.contributor.authorWright, C. A.en_ZA
dc.contributor.authorBezuidenhout, J.en_ZA
dc.contributor.authorSchubert, P. T.en_ZA
dc.date.accessioned2014-07-07T12:10:18Z
dc.date.available2014-07-07T12:10:18Z
dc.date.issued2013
dc.descriptionCITATION: Shibemba, A. L. et al. 2013. An evaluation of the diagnostic adequacy and immunocytochemistry of manual liquid-based smears in breast aspirates. Southern African Journal of Epidemiology and Infection, 28(2):117-121.
dc.descriptionThe original publication is available at http://www.sajei.co.za
dc.description.abstractThe aim of this study was to determine if the Syner-Med®/Cell-Solutions® liquid-based cytology (LBC) technique would provide adequate diagnostic material when applied to breast fine-needle aspiration biopsy (FNAB) specimens and to determine its suitability for immunocytochemistry. A prospective study was undertaken of 38 consecutive patients who underwent FNAB of breast masses in the Fine Needle Aspiration Clinic at Tygerberg Hospital, Cape Town, over a period of six months. Conventional smear cytology slides (CSC) were formulated and the material that remained in the needle was used to prepare the LBC Syner-Med®/Cell-Solutions® slides. The CSC and LBC slides were evaluated by two pathologists. The assessed parameters were cellularity, background and representative diagnostic material. Immunocytochemical stains for pancytokeratin (MNF-116) and oestrogen receptor were performed in each case. In 33 cases (87%), LBC compared favourably with CSC. Adequacy rates of 84.2% for CSC and 76.3% for LBC were found. A diagnosis was made in 78.9% of the CSC cases and in 71% of the LBC cases. The LBC slides showed excellent results, with immunocytochemical staining for MNF-116 and oestrogen receptor. The Syner-Med®/Cell-Solutions® LBC fixative and preparation method provides an alternative technique for obtaining well fixed and prepared slides that are suitable for diagnostic cytology and immunocytochemistry.
dc.description.urihttp://www.sajei.co.za/index.php/SAJEI/article/view/461
dc.description.versionPublisher's version
dc.format.extent5 pages
dc.identifier.citationShibemba, A. L. et al. 2013. An evaluation of the diagnostic adequacy and immunocytochemistry of manual liquid-based smears in breast aspirates. Southern African Journal of Epidemiology and Infection, 28(2):117-121.
dc.identifier.issn2312-0053 (print)
dc.identifier.urihttp://hdl.handle.net/10019.1/91571
dc.languageen_ZA
dc.publisherMedpharm Publications
dc.rights.holderAuthors retain copyright
dc.subjectLiquid-based cytologyen_ZA
dc.subjectBreast -- Needle biopsyen_ZA
dc.subjectBreast -- Cytologyen_ZA
dc.subjectBreast -- Cancer -- Diagnosisen_ZA
dc.subjectImmunocytochemistryen_ZA
dc.titleAn evaluation of the diagnostic adequacy and immunocytochemistry of manual liquid-based smears in breast aspiratesen_ZA
dc.typeArticle
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